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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- TA336949 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal p62/SQSTM1 Antibody (5H7E2)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal p62/SQSTM1 Antibody (5H7E2)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SQSTM1
- Isotype
- IgG
- Antibody clone number
- 5H7E2
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (5H7E2) [TA336949] - Western blot analysis using p62/SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (5H7E2) [TA336949] - Western blot analysis using p62/SQSTM1 mAb in (1) HEK293 and (2) SQSTM1 hIgGFc transfected HEK293 cell lysate.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (5H7E2) [TA336949] - Western blot analysis using p62/SQSTM1 mouse mAb in (1) Hela, (2) Jurkat, (3) THP-1, (4) HEK293, (5) A549, (6) MCF-7, (7) HepG2, (8) COS7 and (9) SK-BR-3 cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [TA336949] - IHC analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [TA336949] - IHC analysis of paraffin-embedded human cervical cancer tissues using p62/SQSTM1 mouse mAb with DAB staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) [TA336949] - p62/SQSTM1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry: p62/SQSTM1 Antibody (5H7E2) [TA336949] - Flow cytometry analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red).
- Validation comment
- FC
