- UM500012 - OriGene SQSTM1 antibody

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Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (Clone UMAB12) at 1:500.
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunofluorescent staining of A549 cells using SQSTM1 mouse monoclonal antibody (UM500012, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunofluorescent staining of HeLa cells using SQSTM1 mouse monoclonal antibody (UM500012, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded colon tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded liver tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded prostate tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human pancreatic cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Reduced cytoplasmic staining is seen in the tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human breast cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human colon cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human colon using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the colon epithelial cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human glioma using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human brain using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the nueral cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human endometrial cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human kidney using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tubule epithelial cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human liver cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human ovarian cancer using anti-SQSTM1 clone UMAB12 mouse monoclonal antibody (UM500012) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.
Validation comment: IHC

UM500012