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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [12]
- Other assay [1]
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- Product number
- UM500013 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#UM500013, RRID:AB_2629028
- Product name
- SQSTM1 mouse monoclonal antibody, clone UMAB13
- Antibody type
- Monoclonal
- Description
- SQSTM1 mouse monoclonal antibody, clone UMAB13
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SQSTM1
- Isotype
- IgG
- Antibody clone number
- UMAB13
- Vial size
- 100 µl
- Concentration
- 0.46 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (Clone UMAB13) at 1:500.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of A549 cells using SQSTM1 mouse monoclonal antibody (UM500013, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using SQSTM1 mouse monoclonal antibody (UM500013, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human liver tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of human liver tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lung tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of human lung tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB13, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human colon cancer using anti-SQSTM1 clone UMAB13 mouse monoclonal antibody (UM500013) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human endometrial cancer using anti-SQSTM1 clone UMAB13 mouse monoclonal antibody (UM500013) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human liver cancer using anti-SQSTM1 clone UMAB13 mouse monoclonal antibody (UM500013) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human ovarian cancer using anti-SQSTM1 clone UMAB13 mouse monoclonal antibody (UM500013) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human glioma using anti-SQSTM1 clone UMAB13 mouse monoclonal antibody (UM500013) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic staining is seen in the tumor cells.
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human brain using anti-SQSTM1 clone UMAB13 mouse monoclonal antibody (UM500013) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the nueral cells.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human colon using anti-SQSTM1 clone UMAB13 mouse monoclonal antibody (UM500013) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong nuclear and cytoplasmic staining is seen in the epithelia cells of colon.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SQSTM1 mouse monoclonal antibody (Clone UMAB13). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-SQSTM1 (Clone UMAB13) very specifically recognizes SQSTM1 antigen on OriGene protein microarray chip.
- Validation comment
- 10K-CHIP
