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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- NBP2-43663 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal p62/SQSTM1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hr and 24hr. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (1478) dilution 1:1000. Beta Actin was used as internal control (bottom panel).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hr and 24hr. HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (1478) dilution 1:1000. Beta Actin was used as internal control (bottom panel).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Analysis of A. 30 ug A549 whole cell lysate/extract B. 30 ug H1299 whole cell lysate/extract C. 30 ug HCT116 whole cell lysate/extract 10 % SDS-PAGE SQSTM1 antibody [1478] dilution: 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (1478) dilution 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p62/SQSTM1 antibody (1478) diluted at 1:500. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Frozen sectioned adult mouse retina. Green: SQSTM1 protein stained by SQSTM1 antibody [1478] diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:250. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: p62/SQSTM1 Antibody (1478) [NBP2-43663] - Analysis of HeLa cell. Brown: Unlabelled sample was also used as a control. Blue: SQSTM1 antibody [1478] dilution: 1:100.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: p62/SQSTM1 Antibody (1478) [NBP2-43663] - An intracellular stain was performed on SK-MEL-28 cells with p62/SQSTM1 Antibody (1478) NBP2-43663APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: p62/SQSTM1 Antibody (1478) [NBP2-43663] - An intracellular stain was performed on U-937 cells with p62/SQSTM1 Antibody (1478) NBP2-43663AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
