LS-C359360

antibody from LSBio

Targeting: SQSTM1 A170, OSIL, p60, p62, p62B, PDB3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C359360

Provider

LSBio

Product name

SQSTM1 Antibody (aa100-400, clone 5H7E2) LS-C359360

Antibody type

Monoclonal

Description

Ammonium sulfate precipitation

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

5H7E2

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot: p62/SQSTM1 Antibody (5H7E2) - Western blot analysis using p62/SQSTM1 mouse mAb in (1) HeLa, (2) Jurkat, (3) THP-1, (4) HEK293, (5) A549, (6) MCF-7, (7) HepG2, (8) COS7 and (9) SK-BR-3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot: p62/SQSTM1 Antibody (5H7E2) - Western blot analysis using p62/SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot: p62/SQSTM1 Antibody (5H7E2) - Western blot analysis using p62/SQSTM1 mAb in (1) HEK293 and (2) SQSTM1 hIgGFc transfected HEK293 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) - p62/SQSTM1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) - IHC analysis of paraffin-embedded human cervical cancer tissues using p62/SQSTM1 mouse mAb with DAB staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) - IHC analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.