Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- LS-C359360 - Provider product page
- Provider
- LSBio
- Product name
- SQSTM1 Antibody (aa100-400, clone 5H7E2) LS-C359360
- Antibody type
- Monoclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5H7E2
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (5H7E2) - Western blot analysis using p62/SQSTM1 mouse mAb in (1) HeLa, (2) Jurkat, (3) THP-1, (4) HEK293, (5) A549, (6) MCF-7, (7) HepG2, (8) COS7 and (9) SK-BR-3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (5H7E2) - Western blot analysis using p62/SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa). This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot: p62/SQSTM1 Antibody (5H7E2) - Western blot analysis using p62/SQSTM1 mAb in (1) HEK293 and (2) SQSTM1 hIgGFc transfected HEK293 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) - p62/SQSTM1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) - IHC analysis of paraffin-embedded human cervical cancer tissues using p62/SQSTM1 mouse mAb with DAB staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry: p62/SQSTM1 Antibody (5H7E2) - IHC analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining. This image was taken for the unconjugated form of this product. Other forms have not been tested.