TA502128

antibody from OriGene

Targeting: SQSTM1 A170, OSIL, p60, p62, p62B, PDB3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502128

Provider

OriGene

Proper citation

OriGene Cat#TA502128, RRID:AB_11124985

Product name

SQSTM1 mouse monoclonal antibody, clone OTI4A12 (formerly 4A12)

Antibody type

Monoclonal

Description

SQSTM1 mouse monoclonal antibody, clone OTI4A12 (formerly 4A12)

Host

Mouse

Conjugate

Unconjugated

Epitope

SQSTM1

Isotype

IgG

Antibody clone number

OTI4A12

Vial size

100 µl

Concentration

0.56 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-SQSTM1 mouse monoclonal antibody (TA502128) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502128)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502128)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502128)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502128)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502128), and then analyzed by flow cytometry.

Validation comment

FC