TA502199

antibody from OriGene

Targeting: SQSTM1 A170, OSIL, p60, p62, p62B, PDB3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502199

Provider

OriGene

Proper citation

OriGene Cat#TA502199, RRID:AB_11126224

Product name

SQSTM1 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)

Antibody type

Monoclonal

Description

SQSTM1 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)

Host

Mouse

Conjugate

Unconjugated

Epitope

SQSTM1

Isotype

IgG

Antibody clone number

OTI1B2

Vial size

100 µl

Concentration

0.57 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502199), and then analyzed by flow cytometry.

Validation comment

FC