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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA502239 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502239, RRID:AB_11140942
- Product name
- SQSTM1 mouse monoclonal antibody, clone OTI6A7 (formerly 6A7)
- Antibody type
- Monoclonal
- Description
- SQSTM1 mouse monoclonal antibody, clone OTI6A7 (formerly 6A7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SQSTM1
- Isotype
- IgG
- Antibody clone number
- OTI6A7
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references The globally disseminated M1T1 clone of group A Streptococcus evades autophagy for intracellular replication.
Barnett TC, Liebl D, Seymour LM, Gillen CM, Lim JY, Larock CN, Davies MR, Schulz BL, Nizet V, Teasdale RD, Walker MJ
Cell host & microbe 2013 Dec 11;14(6):675-82
Cell host & microbe 2013 Dec 11;14(6):675-82
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Western Blot of SQSTM1(p62) protein level by using anti-SQSTM1 antibody in human HEp-2 cell lysates.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SQSTM1 mouse monoclonal antibody (TA502239) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502239), and then analyzed by flow cytometry.
- Validation comment
- FC
