Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [3]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-27800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SQSTM1 Monoclonal Antibody (GT1478)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: HepG2, A549, H1299, HCT116, Huh-7 (untreated), Huh-7 (3 µM Thapsigargin treatment for 12 hr), Huh-7 (3 µM Thapsigargin treatment for 24 hr), HepG2 (3 µM Thapsigargin treatment for 12 hr), HepG2 (3 µM Thapsigargin treatment for 24 hr), 2C4, MEF, MNT-1 Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT1478
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Influences of cyclosporin A and non-immunosuppressive derivatives on cellular cyclophilins and viral nucleocapsid protein during human coronavirus 229E replication.
Ma-Lauer Y, Zheng Y, Malešević M, von Brunn B, Fischer G, von Brunn A
Antiviral research 2020 Jan;173:104620
Antiviral research 2020 Jan;173:104620
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SQSTM1 was performed in HepG2 cells treated with 3 µM thapsigargin 16 hrs (rigtht) and mock (left) fixed in ice-cold MeOH for 10 Min, permeabilize with cooled acetone for 1 min . Green: SQSTM1 Monoclonal Antibody (GT1478) (Product # MA5-27800) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SQSTM1 antibody [GT1478] detects SQSTM1 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50 µM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (Product # MA5-27800) diluted at 1:1,000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of SQSTM1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SQSTM1 Monoclonal Antibody (GT1478) (Product # MA5-27800) at 1:500 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing cytoplasm, endosome-like, plasma membrane and nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SQSTM1 antibody [GT1478] detects SQSTM1 protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (Product # MA5-27800) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of SQSTM1 in HeLa cells. Samples were treated with SQSTM1 monoclonal antibody (Product # MA5-27800) using a dilution of 1:100. Results are as follows: Brown: Unlabelled sample was also used as a control, Blue: SQSTM1 antibody. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter..
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SQSTM1 antibody [GT1478] (Product # MA5-27800) detects SQSTM1 protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4°C for 5 min. Brown: Unlabelled sample was also used as a control. Blue: SQSTM1 antibody [GT1478] (Product # MA5-27800) dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SQSTM1 antibody [GT1478] (Product # MA5-27800) detects SQSTM1 protein by flow cytometry analysis. Sample: HeLa cell fixed in 4% paraformaldehyde at 4°C for 5 min. Brown: Unlabelled sample was also used as a control. Blue: SQSTM1 antibody [GT1478] (Product # MA5-27800) dilution: 1:100. Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Co-immunostaining of CypB and cell organelles in HCoV-229E-infected Huh7 cells . For the identification of the intense cyclophilin B bleb-like structures infected cells (MOI = 1; 48 h p.i.) were co-stained with anti-CypB and antibodies directed against markers of the ER (anti-PDI), cis-GOLGI (anti-GM130), autophagosomes (anti-SQSTM1), anti-P-bodies (anti-hDcp1a) and stress granules (anti-PABP). Cyclophilin B normally distributes within the ER. In the presence of HCoV-229E, it intriguingly concentrates at bleb-like structures of the ER. Fig. 6
