NBP1-48320

Selka A, Doiron JA, Lyons P, Dastous S, Chiasson A, Cormier M, Turcotte S, Surette ME, Touaibia M
European journal of medicinal chemistry 2019 Oct 1;179:347-357

Lipopolysaccharide enhances DNA-induced IFN-β expression and autophagy by upregulating cGAS expression in A549 cells.

Wang R, Wang W, Li A, Wang Y, Jin J, Huang Z, Huang G
Experimental and therapeutic medicine 2019 Nov;18(5):4157-4164

Transgenic expression of a ratiometric autophagy probe specifically in neurons enables the interrogation of brain autophagy in vivo.

Lee JH, Rao MV, Yang DS, Stavrides P, Im E, Pensalfini A, Huo C, Sarkar P, Yoshimori T, Nixon RA
Autophagy 2019 Mar;15(3):543-557

Cabrera JR, Manivanh R, North BJ, Leib DA
mBio 2019 Mar 5;10(2)

Decorin Expression in Human Vulva Carcinoma: Oncosuppressive Effect of Decorin cDNA Transduction on Carcinoma Cells.

Nyman MC, Jokilammi AB, Boström PC, Kurki SH, Sainio AO, Grenman SE, Orte KJ, Hietanen SH, Elenius K, Järveläinen HT
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2019 Jul;67(7):511-522

MicroRNA-124 regulates the expression of p62/p38 and promotes autophagy in the inflammatory pathogenesis of Parkinson's disease.

Yao L, Zhu Z, Wu J, Zhang Y, Zhang H, Sun X, Qian C, Wang B, Xie L, Zhang S, Lu G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jul;33(7):8648-8665

The activation of Mucolipin TRP channel 1 (TRPML1) protects motor neurons from L-BMAA neurotoxicity by promoting autophagic clearance.

Tedeschi V, Petrozziello T, Sisalli MJ, Boscia F, Canzoniero LMT, Secondo A
Scientific reports 2019 Jul 24;9(1):10743

Shifting the balance of autophagy and proteasome activation reduces proteotoxic cell death: a novel therapeutic approach for restoring photoreceptor homeostasis.

Qiu Y, Yao J, Jia L, Thompson DA, Zacks DN
Cell death & disease 2019 Jul 18;10(8):547

17β-Estradiol protects mesenchymal stem cells against high glucose-induced mitochondrial oxidants production via Nrf2/Sirt3/MnSOD signaling.

Oh JY, Choi GE, Lee HJ, Jung YH, Chae CW, Kim JS, Lee CK, Han HJ
Free radical biology & medicine 2019 Jan;130:328-342

Autophagosome immunoisolation from GFP-LC3B mouse tissue.

Yao J, Qiu Y, Jia L, Zacks DN
Autophagy 2019 Feb;15(2):341-346

Exercise regulates lipid droplet dynamics in normal and fatty liver.

la Fuente FP, Quezada L, Sepúlveda C, Monsalves-Alvarez M, Rodríguez JM, Sacristán C, Chiong M, Llanos M, Espinosa A, Troncoso R
Biochimica et biophysica acta. Molecular and cell biology of lipids 2019 Dec;1864(12):158519

Inhibiting autophagy reduces retinal degeneration caused by protein misfolding.

Yao J, Qiu Y, Frontera E, Jia L, Khan NW, Klionsky DJ, Ferguson TA, Thompson DA, Zacks DN
Autophagy 2018;14(7):1226-1238

CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation.

Mishra SK, Gao YG, Deng Y, Chalfant CE, Hinchcliffe EH, Brown RE
Autophagy 2018;14(5):862-879

Inhibition of WNT-CTNNB1 signaling upregulates SQSTM1 and sensitizes glioblastoma cells to autophagy blockers.

Nàger M, Sallán MC, Visa A, Pushparaj C, Santacana M, Macià A, Yeramian A, Cantí C, Herreros J
Autophagy 2018;14(4):619-636

Critical Role of Beclin1 in HIV Tat and Morphine-Induced Inflammation and Calcium Release in Glial Cells from Autophagy Deficient Mouse.

Lapierre J, Rodriguez M, Ojha CR, El-Hage N
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 2018 Sep;13(3):355-370

Metformin exacerbates and simvastatin attenuates myelin damage in high fat diet-fed C57BL/6 J mice.

Ciric D, Martinovic T, Petricevic S, Trajkovic V, Bumbasirevic V, Kravic-Stevovic T
Neuropathology : official journal of the Japanese Society of Neuropathology 2018 Oct;38(5):468-474

Glucocorticoid-mediated ER-mitochondria contacts reduce AMPA receptor and mitochondria trafficking into cell terminus via microtubule destabilization.

Choi GE, Oh JY, Lee HJ, Chae CW, Kim JS, Jung YH, Han HJ
Cell death & disease 2018 Nov 14;9(11):1137

Complement C3 Drives Autophagy-Dependent Restriction of Cyto-invasive Bacteria.

Sorbara MT, Foerster EG, Tsalikis J, Abdel-Nour M, Mangiapane J, Sirluck-Schroeder I, Tattoli I, van Dalen R, Isenman DE, Rohde JR, Girardin SE, Philpott DJ
Cell host & microbe 2018 May 9;23(5):644-652.e5

Autophagy-independent increase of ATG5 expression in T cells of multiple sclerosis patients.

Paunovic V, Petrovic IV, Milenkovic M, Janjetovic K, Pravica V, Dujmovic I, Milosevic E, Martinovic V, Mesaros S, Drulovic J, Trajkovic V
Journal of neuroimmunology 2018 Jun 15;319:100-105

microRNA-199a/b-5p enhance imatinib efficacy via repressing WNT2 signaling-mediated protective autophagy in imatinib-resistant chronic myeloid leukemia cells.

Chen PH, Liu AJ, Ho KH, Chiu YT, Anne Lin ZH, Lee YT, Shih CM, Chen KC
Chemico-biological interactions 2018 Aug 1;291:144-151

Phellinus linteus Mycelium Alleviates Myocardial Ischemia-Reperfusion Injury through Autophagic Regulation.

Su HH, Chu YC, Liao JM, Wang YH, Jan MS, Lin CW, Wu CY, Tseng CY, Yen JC, Huang SS
Frontiers in pharmacology 2017;8:175

Involvement of proapoptotic genes in autophagic cell death induced by irradiation.

Song J, Zhao X, Feng Y, Xu S, Zhang Y, Wei L
Cell death discovery 2017;3:17068

Amyloid β1-42 (Aβ1-42) Induces the CDK2-Mediated Phosphorylation of Tau through the Activation of the mTORC1 Signaling Pathway While Promoting Neuronal Cell Death.

Lee KH, Lee SJ, Lee HJ, Choi GE, Jung YH, Kim DI, Gabr AA, Ryu JM, Han HJ
Frontiers in molecular neuroscience 2017;10:229

Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy.

Shen L, Yang Y, Ou T, Key CC, Tong SH, Sequeira RC, Nelson JM, Nie Y, Wang Z, Boudyguina E, Shewale SV, Zhu X
Journal of lipid research 2017 Sep;58(9):1808-1821

Pimozide reduces toxic forms of tau in TauC3 mice via 5' adenosine monophosphate-activated protein kinase-mediated autophagy.

Kim YD, Jeong EI, Nah J, Yoo SM, Lee WJ, Kim Y, Moon S, Hong SH, Jung YK
Journal of neurochemistry 2017 Sep;142(5):734-746

Neither eosinophils nor neutrophils require ATG5-dependent autophagy for extracellular DNA trap formation.

Germic N, Stojkov D, Oberson K, Yousefi S, Simon HU
Immunology 2017 Nov;152(3):517-525

Vps34 PI 3-kinase inactivation enhances insulin sensitivity through reprogramming of mitochondrial metabolism.

Bilanges B, Alliouachene S, Pearce W, Morelli D, Szabadkai G, Chung YL, Chicanne G, Valet C, Hill JM, Voshol PJ, Collinson L, Peddie C, Ali K, Ghazaly E, Rajeeve V, Trichas G, Srinivas S, Chaussade C, Salamon RS, Backer JM, Scudamore CL, Whitehead MA, Keaney EP, Murphy LO, Semple RK, Payrastre B, Tooze SA, Vanhaesebroeck B
Nature communications 2017 Nov 27;8(1):1804

Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer.

Eritja N, Chen BJ, Rodríguez-Barrueco R, Santacana M, Gatius S, Vidal A, Martí MD, Ponce J, Bergadà L, Yeramian A, Encinas M, Ribera J, Reventós J, Boyd J, Villanueva A, Matias-Guiu X, Dolcet X, Llobet-Navàs D
Autophagy 2017 Mar 4;13(3):608-624

Human Astrocytes Transfer Aggregated Alpha-Synuclein via Tunneling Nanotubes.

Rostami J, Holmqvist S, Lindström V, Sigvardson J, Westermark GT, Ingelsson M, Bergström J, Roybon L, Erlandsson A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Dec 6;37(49):11835-11853

A pilot study of an autologous tumor-derived autophagosome vaccine with docetaxel in patients with stage IV non-small cell lung cancer.

Sanborn RE, Ross HJ, Aung S, Acheson A, Moudgil T, Puri S, Hilton T, Fisher B, Coffey T, Paustian C, Neuberger M, Walker E, Hu HM, Urba WJ, Fox BA
Journal for immunotherapy of cancer 2017 Dec 19;5(1):103

Impaired endo-lysosomal membrane integrity accelerates the seeding progression of α-synuclein aggregates.

Jiang P, Gan M, Yen SH, McLean PJ, Dickson DW
Scientific reports 2017 Aug 9;7(1):7690

TBC1D20 mediates autophagy as a key regulator of autophagosome maturation.

Sidjanin DJ, Park AK, Ronchetti A, Martins J, Jackson WT
Autophagy 2016 Oct 2;12(10):1759-1775

Aβ-Induced Drp1 phosphorylation through Akt activation promotes excessive mitochondrial fission leading to neuronal apoptosis.

Kim DI, Lee KH, Gabr AA, Choi GE, Kim JS, Ko SH, Han HJ
Biochimica et biophysica acta 2016 Nov;1863(11):2820-2834

Herpes Simplex Virus and Interferon Signaling Induce Novel Autophagic Clusters in Sensory Neurons.

Katzenell S, Leib DA
Journal of virology 2016 May;90(9):4706-4719

Vibrio vulnificus VvhA induces autophagy-related cell death through the lipid raft-dependent c-Src/NOX signaling pathway.

Song EJ, Lee SJ, Lim HS, Kim JS, Jang KK, Choi SH, Han HJ
Scientific reports 2016 Jun 2;6:27080

Hinokitiol induces autophagy in murine breast and colorectal cancer cells.

Wang WK, Lin ST, Chang WW, Liu LW, Li TY, Kuo CY, Hsieh JL, Lee CH
Environmental toxicology 2016 Jan;31(1):77-84

Defective Acetylcholine Receptor Subunit Switch Precedes Atrophy of Slow-Twitch Skeletal Muscle Fibers Lacking ERK1/2 Kinases in Soleus Muscle.

Wang S, Seaberg B, Paez-Colasante X, Rimer M
Scientific reports 2016 Dec 9;6:38745

Fucosylation of LAMP-1 and LAMP-2 by FUT1 correlates with lysosomal positioning and autophagic flux of breast cancer cells.

Tan KP, Ho MY, Cho HC, Yu J, Hung JT, Yu AL
Cell death & disease 2016 Aug 25;7(8):e2347

Differing roles of autophagy in HIV-associated neurocognitive impairment and encephalitis with implications for morphine co-exposure.

Dever SM, Rodriguez M, Lapierre J, Costin BN, El-Hage N
Frontiers in microbiology 2015;6:653

Deletion of autophagy inducer RB1CC1 results in degeneration of the retinal pigment epithelium.

Yao J, Jia L, Khan N, Lin C, Mitter SK, Boulton ME, Dunaief JL, Klionsky DJ, Guan JL, Thompson DA, Zacks DN
Autophagy 2015;11(6):939-53

The generation of neutrophils in the bone marrow is controlled by autophagy.

Rožman S, Yousefi S, Oberson K, Kaufmann T, Benarafa C, Simon HU
Cell death and differentiation 2015 Mar;22(3):445-56

In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy.

Lin HH
Chemical research in toxicology 2015 Feb 16;28(2):202-15

Neurite Aggregation and Calcium Dysfunction in iPSC-Derived Sensory Neurons with Parkinson's Disease-Related LRRK2 G2019S Mutation.

Schwab AJ, Ebert AD
Stem cell reports 2015 Dec 8;5(6):1039-1052

Tumor growth suppression by inhibiting both autophagy and STAT3 signaling in HNSCC.

Fan TF, Bu LL, Wang WM, Ma SR, Liu JF, Deng WW, Mao L, Yu GT, Huang CF, Liu B, Zhang WF, Sun ZJ
Oncotarget 2015 Dec 22;6(41):43581-93

Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine.

Lin CW, Jan MS, Kuo JH
Molecular pharmaceutics 2015 Aug 3;12(8):2858-68

MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation.

Sun KT, Chen MY, Tu MG, Wang IK, Chang SS, Li CY
Bone 2015 Apr;73:145-53

A dual role for autophagy in a murine model of lung cancer.

Rao S, Tortola L, Perlot T, Wirnsberger G, Novatchkova M, Nitsch R, Sykacek P, Frank L, Schramek D, Komnenovic V, Sigl V, Aumayr K, Schmauss G, Fellner N, Handschuh S, Glösmann M, Pasierbek P, Schlederer M, Resch GP, Ma Y, Yang H, Popper H, Kenner L, Kroemer G, Penninger JM
Nature communications 2014;5:3056

Vacuolin-1 potently and reversibly inhibits autophagosome-lysosome fusion by activating RAB5A.

Lu Y, Dong S, Hao B, Li C, Zhu K, Guo W, Wang Q, Cheung KH, Wong CW, Wu WT, Markus H, Yue J
Autophagy 2014;10(11):1895-905

Lin CW, Jan MS, Kuo JH
Molecular pharmaceutics 2014 Sep 2;11(9):3002-8

Rapamycin improves peripheral nerve myelination while it fails to benefit neuromuscular performance in neuropathic mice.

Nicks J, Lee S, Harris A, Falk DJ, Todd AG, Arredondo K, Dunn WA Jr, Notterpek L
Neurobiology of disease 2014 Oct;70:224-36

microRNA-18a induces apoptosis in colon cancer cells via the autophagolysosomal degradation of oncogenic heterogeneous nuclear ribonucleoprotein A1.

Fujiya M, Konishi H, Mohamed Kamel MK, Ueno N, Inaba Y, Moriichi K, Tanabe H, Ikuta K, Ohtake T, Kohgo Y
Oncogene 2014 Oct 2;33(40):4847-56

Salmonella induce autophagy in melanoma by the downregulation of AKT/mTOR pathway.

Lee CH, Lin ST, Liu JJ, Chang WW, Hsieh JL, Wang WK
Gene therapy 2014 Mar;21(3):309-16

Overexpression of Atg5 in mice activates autophagy and extends lifespan.

Pyo JO, Yoo SM, Ahn HH, Nah J, Hong SH, Kam TI, Jung S, Jung YK
Nature communications 2013;4:2300

Bortezomib induces apoptosis and autophagy in osteosarcoma cells through mitogen-activated protein kinase pathway in vitro.

Lou Z, Ren T, Peng X, Sun Y, Jiao G, Lu Q, Zhang S, Lu X, Guo W
The Journal of international medical research 2013 Oct;41(5):1505-19

Knockdown of core binding factorβ alters sphingolipid metabolism.

Greer AH, Yong T, Fennell K, Moustafa YW, Fowler M, Galiano F, Ng SW, Berkowitz RS, Cardelli J, Meyers S, Davis JN
Journal of cellular physiology 2013 Dec;228(12):2350-64

Vitamin C: a novel regulator of neutrophil extracellular trap formation.

Mohammed BM, Fisher BJ, Kraskauskas D, Farkas D, Brophy DF, Fowler AA 3rd, Natarajan R
Nutrients 2013 Aug 9;5(8):3131-51

Herpes simplex virus γ34.5 interferes with autophagosome maturation and antigen presentation in dendritic cells.

Gobeil PA, Leib DA
mBio 2012 Oct 16;3(5):e00267-12

Protective role of autophagy in branched polyethylenimine (25K)- and poly(L-lysine) (30-70K)-induced cell death.

Lin CW, Jan MS, Kuo JH, Hsu LJ, Lin YS
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2012 Dec 18;47(5):865-74

p53/HMGB1 complexes regulate autophagy and apoptosis.

Livesey KM, Kang R, Vernon P, Buchser W, Loughran P, Watkins SC, Zhang L, Manfredi JJ, Zeh HJ 3rd, Li L, Lotze MT, Tang D
Cancer research 2012 Apr 15;72(8):1996-2005

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: p62/SQSTM1 Antibody [NBP1-48320] - Lane view shows a specific band for p62/SQSTM1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: p62/SQSTM1 Antibody [NBP1-48320] - Cultured HeLa cells were treated with or without 50 uM chloriquine for 24 hours as indicated. Cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 1 ug/ml anti-p62/SQSMT1 in 1% milk and dectected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.