Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-12092 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SQSTM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SQSTM1 (arrow) using a SQSTM1 polyclonal antibody (Product # PA5-12092) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of pre-incubated without (lane 1) and with (lane 2) blocking peptide in MCF-7 cell lysate using a SQSTM1 polyclonal antibody (Product # PA5-12092).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis using a SQSTM1 polyclonal antibody (Product # PA5-12092)in Methanol-fixed and PFA fixed HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells using a SQSTM1 polyclonal antibody (Product # PA5-12092). U251 cells were treated with Chloroquine (50 uM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), cells were then incubated with a SQSTM1 polyclonal antibody (Product # PA5-12092) (1:200, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells using a SQSTM1 polyclonal antibody (Product # PA5-12092). U251 cells were treated with Chloroquine (50 uM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), cells were then incubated with a SQSTM1 polyclonal antibody (Product # PA5-12092) (1:200, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a SQSTM1 polyclonal antibody (Product # PA5-12092), followed by HRP-conjugated secondary antibody and DAB staining.