LS-B1454

antibody from LSBio

Targeting: SERPINA1 A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

SERPINA1 protein structure - LS-B1454 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Alpha-1-Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Goat-anti-Alpha-1-Anti-Trypsin (LS-C59210 lot 5842, red), Rabbit anti-Transferrin (LS-C59393 lot 3033), and Mouse-a-GST (LS-C19065 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (green) DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the Bio-Rad VersaDoc System.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Alpha-1anti-Trypsin Polyclonal Antibody-Western blot. Goat anti-Alpha-1anti-Trypsin antibody was used to detect Alpha-1anti-Trypsin under reducing conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti-goat (1:10K in TBS/MB-070 1 hr RT). Images were collected using the Bio-Rad VersaDoc System.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Alpha 1 anti-trypsin antibody-Fluorescent western blot. Primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (LS-C59393, green); Alpha 1 anti-trypsin (LS-C59210, red); and Human IgG (LS-C59376, Blue) in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was washed 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Bio-Rad VersaDoc4000.