16382-1-AP

antibody from Proteintech Group

Targeting: SERPINA1 A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1

Provider product page for 16382-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [42]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 16382-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#16382-1-AP, RRID:AB_10641185
Product name: Alpha 1 Antitrypsin antibody
Antibody type: Polyclonal
Description: Alpha 1 Antitrypsin antibody (Cat. #16382-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SERPINA1 protein structure - 16382-1-AP shown in red.

Submitted references Cytosine base editing of LPA in transgenic mice averts large deletions.

Chuecos MA, Park SH, Bhakta MM, Too-Chiobi U, Betancourth D, Cao M, De Giorgi M, Walkey CJ, Tiwari A, Godin B, Assini JM, Palmer DJ, Ng P, Boffa MB, Koschinsky ML, Bao G, Lagor WR
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Cell 2026 Mar 19;189(6):1768-1784.e24

Terpinen-4-ol suppresses proliferation and motility of cutaneous squamous cell carcinoma cells by enhancing calpain-2 expression.

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Identification of Anoikis-Related Genes in Driving Immune-Inflammatory Responses in Ulcerative Colitis Based on Bioinformatics Analysis and Machine Learning.

Diao W, Huang X, Huang W, Jiang J, Li W, Liu H, Yan B, Shen L
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Galectin-8 binding to alpha-1 antitrypsin is a physiological mechanism in healthy individuals but exacerbates the symptoms of alpha-1 antitrypsin deficiency.

Sayed H, Mayo KH, Zhou Y, Tai G, Su J
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Li J, Liu C, Fu Z, He Q, Liu X, Sun Z
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Oligoclonal tumor-specific CD8 T-cell revival and IRE1α/XBP1-GDF15-mediated immunosuppressive niches determine neoadjuvant chemoimmunotherapy efficacy in cervical cancer.

Cao G, Wang Y, Zeng H, Zhi Y, Guo Y, Xu M, Ruan Y, Wang Y, Xiao Y, Lu J, Tse KY, Gao J, Zhang Q, Wang C, Han Z, Li F
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Li J, Huang H, Yin Y, Mao Y, Li M, Li H, Jiang C, Yang R
Clinical epigenetics 2025 May 29;17(1):86

Bisphenol A exposure enhances proliferation and tumorigenesis of papillary thyroid carcinoma through ROS generation and activation of NOX4 signaling pathways.

Wang Y, Su X, Wang Q, Zhang L, Yu Y, Zhao Y, Liu Z
Ecotoxicology and environmental safety 2025 Mar 1;292:117946

Expression and purification of recombinant glutaredoxin 1 and protection against oxidative stress injury during cerebral ischemia-reperfusion injury.

Li ZT, Lin T, Sun Y, Wang XY, Yang YX, Gan L, Xu JM, Wei XT, Zhu HQ, Zhao WC, Zhu ZH
Protein expression and purification 2025 Jun;230:106689

Multi-omics analysis reveals metabolic disruptions in cardiac tissues of aging nonhuman primates with spontaneous type 2 diabetes.

Liu Y, Yu J, Hu H, Pu S, Wu H, Ma Y, Yang W, Fang C, Sun F, Wang H
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snoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification.

Liu B, Wu T, Miao BA, Ji F, Liu S, Wang P, Zhao Y, Zhong Y, Sundaram A, Zeng TB, Majcherska-Agrawal M, Keenan RJ, Pan T, He C
Cell 2025 Jan 23;188(2):465-483.e22

Proteomic analysis of spinal dorsal horn in prior exercise protection against neuropathic pain.

Chen B, Guo J, Gong C, Zhu C, Wu Y, Wang S, Zheng Y, Lu H
Scientific reports 2025 Jan 18;15(1):2391

Alpha-1 Antitrypsin Mediates Spontaneous Resolution of Acute Gouty Inflammation Via Inhibiting Caspase 3/GSDME-dependent Macrophage Pyroptosis.

Li X, Liu L, Tong S, Wang F, Zhu L, Cheng Q, Zhang P, Chen M, Xie Y, Wang L, Yu H, Wu H
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LGR6 modulates intervertebral disc degeneration through regulation of macrophage efferocytosis.

Li F, Shi Y, Chen J, Sun J, Shi J, Sun K, Zheng B
Journal of translational medicine 2025 Apr 25;23(1):475

Alpha-1 antitrypsin inhibits Clostridium botulinum C2 toxin, Corynebacterium diphtheriae diphtheria toxin and B. anthracis fusion toxin.

Lietz S, Sokolowski LM, Barth H, Ernst K
Scientific reports 2024 Sep 11;14(1):21257

Regulation of hepatic inclusions and fibrinogen biogenesis by SEL1L-HRD1 ERAD.

Song Z, Thepsuwan P, Hur WS, Torres M, Wu SA, Wei X, Tushi NJ, Wei J, Ferraresso F, Paton AW, Paton JC, Zheng Z, Zhang K, Fang D, Kastrup CJ, Jaiman S, Flick MJ, Sun S
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CEBPB-mediated upregulation of SERPINA1 promotes colorectal cancer progression by enhancing STAT3 signaling.

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Cell death discovery 2024 May 6;10(1):219

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Lietz S, Sommer A, Sokolowski LM, Kling C, Rodríguez Alfonso AA, Preising N, Alpízar-Pedraza D, King J, Streit L, Schröppel B, van Erp R, Barth E, Schneider M, Münch J, Michaelis J, Ständker L, Wiese S, Barth H, Pulliainen AT, Scanlon K, Ernst K
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Yang J, Zhi Y, Wen S, Pan X, Wang H, He X, Lu Y, Zhu Y, Chen Y, Shi G
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Vesicular Integral-Membrane Protein 36 Is Involved in the Selective Secretion of Fucosylated Proteins into Bile Duct-like Structures in HepG2 Cells.

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International journal of molecular sciences 2023 Apr 11;24(8)

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Ouni E, Nedbal V, Da Pian M, Cao H, Haas KT, Peaucelle A, Van Kerk O, Herinckx G, Marbaix E, Dolmans MM, Tuuri T, Otala M, Amorim CA, Vertommen D
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Nutrients 2022 Jun 1;14(11)

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Kaserman JE, Werder RB, Wang F, Matte T, Higgins MI, Dodge M, Lindstrom-Vautrin J, Bawa P, Hinds A, Bullitt E, Caballero IS, Shi X, Gerszten RE, Brunetti-Pierri N, Liesa M, Villacorta-Martin C, Hollenberg AN, Kotton DN, Wilson AA
Cell reports 2022 Dec 6;41(10):111775

Knockdown of Alpha-1 Antitrypsin with antisense oligonucleotide does not exacerbate smoke induced lung injury.

Stearns K, Goldklang M, Xiao R, Zelonina T, Blomenkamp K, Teckman J, D'Armiento JM
PloS one 2021;16(2):e0246040

Alpha-1 antitrypsin inhibits TMPRSS2 protease activity and SARS-CoV-2 infection.

Wettstein L, Weil T, Conzelmann C, Müller JA, Groß R, Hirschenberger M, Seidel A, Klute S, Zech F, Prelli Bozzo C, Preising N, Fois G, Lochbaum R, Knaff PM, Mailänder V, Ständker L, Thal DR, Schumann C, Stenger S, Kleger A, Lochnit G, Mayer B, Ruiz-Blanco YB, Hoffmann M, Sparrer KMJ, Pöhlmann S, Sanchez-Garcia E, Kirchhoff F, Frick M, Münch J
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De Giorgi M, Li A, Hurley A, Barzi M, Doerfler AM, Cherayil NA, Smith HE, Brown JD, Lin CY, Bissig KD, Bao G, Lagor WR
Molecular therapy. Methods & clinical development 2021 Jun 11;21:656-669

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Cui C, Chakraborty K, Tang XA, Zhou G, Schoenfelt KQ, Becker KM, Hoffman A, Chang YF, Blank A, Reardon CA, Kenny HA, Vaisar T, Lengyel E, Greene G, Becker L
Cell 2021 Jun 10;184(12):3163-3177.e21

Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.

Li X, Huang C, Zhang X, Yang T, Zuo S, Fu C, Zhang Y, Yang C, Chen L
Translational andrology and urology 2021 Aug;10(8):3402-3414

Alpha-1 Antitrypsin Induces Epithelial-to-Mesenchymal Transition, Endothelial-to-Mesenchymal Transition, and Drug Resistance in Lung Cancer Cells.

Wu DM, Liu T, Deng SH, Han R, Zhang T, Li J, Xu Y
OncoTargets and therapy 2020;13:3751-3763

Altered phenotype in LMAN1-deficient mice with low levels of residual LMAN1 expression.

Everett LA, Khoriaty RN, Zhang B, Ginsburg D
Blood advances 2020 Nov 24;4(22):5635-5643

Proteomic analysis reveals the protective effects of emodin on severe acute pancreatitis induced lung injury by inhibiting neutrophil proteases activity.

Xu C, Zhang J, Liu J, Li Z, Liu Z, Luo Y, Xu Q, Wang M, Zhang G, Wang F, Chen H
Journal of proteomics 2020 May 30;220:103760

Comparative analysis of proteomes between diabetic and normal human sperm: Insights into the effects of diabetes on male reproduction based on the regulation of mitochondria-related proteins.

An T, Wang YF, Liu JX, Pan YY, Liu YF, He ZC, Mo FF, Li J, Kang LH, Gu YJ, Lv BH, Gao SH, Jiang GJ
Molecular reproduction and development 2018 Jan;85(1):7-16

Free circulating active elastase contributes to chronic inflammation in patients on hemodialysis.

Khatib-Massalha E, Michelis R, Trabelcy B, Gerchman Y, Kristal B, Ariel A, Sela S
American journal of physiology. Renal physiology 2018 Feb 1;314(2):F203-F209

Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection.

Jiao F, Zhang D, Jiang M, Mi J, Liu X, Zhang H, Hu Z, Xu X, Hu X
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Mobin MB, Gerstberger S, Teupser D, Campana B, Charisse K, Heim MH, Manoharan M, Tuschl T, Stoffel M
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Ludwig MR, Kojima K, Bowersock GJ, Chen D, Jhala NC, Buchsbaum DJ, Grizzle WE, Klug CA, Mobley JA
Proteomics 2016 Feb;16(3):516-31

Identification of differentially expressed serum proteins in infectious purpura fulminans.

He T, Hu JY, Han J, Zhang DX, Jiang XP, Chen B, Huang YS
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High-throughput screening of tumor metastatic-related differential glycoprotein in hepatocellular carcinoma by iTRAQ combines lectin-related techniques.

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse liver tissue were subjected to SDS PAGE followed by western blot with 16382-1-AP(Alpha-1-Antitrypsin antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The Alpha-1-Antitrypsin antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Alpha-1-Antitrypsin. This antibody recognizes human,mouse,rat antigen. The Alpha-1-Antitrypsin antibody has been validated for the following applications: ELISA, IHC, WB analysis.
Appendix: 16382-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver using 16382-1-AP(Alpha-1-Antitrypsin antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue