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LearnAF2979
antibody from Novus Biologicals
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- AF2979 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Serpin A1c/alpha 1-Antitrypsin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Serpin A1c/alpha 1-Antitrypsin in direct ELISAs and Western blots. In direct ELISAs, approximately 70% cross-reactivity with recombinant mouse Serpin A1a is observed, and less than 3% cross-reactivity with recombinant human Serpin A1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A combined in silico and in vitro study on mouse Serpina1a antitrypsin-deficiency mutants.
Eggenschwiler R, Patronov A, Hegermann J, Fráguas-Eggenschwiler M, Wu G, Cortnumme L, Ochs M, Antes I, Cantz T
Scientific reports 2019 May 16;9(1):7486
Scientific reports 2019 May 16;9(1):7486
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Serpin A1c/alpha 1-Antitrypsin by Simple WesternTM. Simple Western lane view shows mouse serum and lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1c/alpha 1-Antitrypsin at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Serpin A1c/alpha 1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2979) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Serpin A1c/alpha 1-Antitrypsin by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Serpin A1c/alpha 1-Antitrypsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2979) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin A1c/alpha 1-Antitrypsin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
