Explore
Validate
LearnTA319138
antibody from OriGene
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA319138 - Provider product page
- Provider
- OriGene
- Product name
- Goat polyclonal Alpha-1-Anti-Trypsin antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal Alpha-1-Anti-Trypsin antibody
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- SERPINA1
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent WB. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used to detect target proteins under reducing conditions in albumin depleted human serum. Primary antibody: Each primary antibody at 1:1000 . Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot of Goat anti Alpha-1anti-Trypsin antibody. Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 55kDa for a-1 Anti-Trypsin. Other band(s): none.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB of goat anti-Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent WB of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4°C.
- Validation comment
- WB
