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LearnNB120-7635-0.1mg
antibody from Novus Biologicals
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
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- Product number
- NB120-7635-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Serpin A1/alpha 1-Antitrypsin Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. Alpha 1 Antitrypsin
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [HRP] [NB120-7635] - Lane 1: molecular weight. Lane 2: Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST. Load: 35 ug per lane under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Primary antibody: Alpha 1 Anti-Trypsin antibody at 1:400 for overnight at 4C. Secondary antibody: DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT in incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): DyLight 488 Donkey anti-Mouse IgG (blue) and DyLight549 Donkey anti-Rabbit IgG (green) secondary antibodies.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [HRP] [NB120-7635] - Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.
