GTX48494

antibody from GeneTex

Targeting: SERPINA1 A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

GTX48494

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48494, RRID:AB_11174952

Product name

alpha 1 Antitrypsin antibody

Antibody type

Polyclonal

Antigen

Other

Host

Goat

Isotype

IgG

Vial size

1ml

Storage

Store vial at 4° C for short-term use.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Goat-anti-Alpha-1-Anti-Trypsin (GTX48494, red), Rabbit anti-Transferrin , and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in blocking buffer over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (shown as green)   DyLight 488 Donkey anti-Mouse IgG (shown as blue),  and DyLight 649 Donkey anti-Goat IgG (shown as red) at 1:10000 (in blocking buffer min RT). Blots were washed, rinsed in methanol, and dried

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Goat anti Alpha-1anti-Trypsin antibody (GTX48494) was used to detect Alpha-1anti-Trypsin under reducing (R) conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (on 4 C in blocking buffer). Detection shown was using Dylight 488 conjugated Donkey anti goat ( 1:10K in TBS/blocking buffer 1 hr RT).

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin 1°, green 2°); Alpha 1 anti trypsin (1° GTX48494, red 2° Goat IgG DyLight™ 549 Conjugated); and Human IgG 1°, Blue 2° Mouse IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~ 1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged.

Validation comment

WB