Explore
Validate
LearnGTX83663
antibody from GeneTex
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX83663 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX83663, RRID:AB_10729403
- Product name
- alpha 1 Antitrypsin antibody [11G2]
- Antibody type
- Monoclonal
- Reactivity
- Human, Canine, Simian
- Host
- Mouse
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of A1AT by using TrueMab monoclonal anti-A1AT antibodies (Negative control: IP without adding anti-A1AT antibody.). For each experiment, 500ul of DDK tagged A1AT overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-A1AT antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-SERPINA1mouse monoclonal antibody. (GTX83663, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-SERPINA1mouse monoclonal antibody. (GTX83663, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-SERPINA1mouse monoclonal antibody. (GTX83663, Dilution 1:50)
