MAB1268-100

antibody from R&D Systems

Targeting: SERPINA1 A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

MAB1268-100

Provider

R&D Systems

Product name

Human Serpin A1/alpha 1-Antitrypsin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Serpin A1/alpha 1-Antitrypsin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Serpin A3, A4, A5, C1, F1, recombinant mouse Serpin D1, or F2 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P01009


Isotype

IgG

Antibody clone number

202808

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Modified serpinA1 as risk marker for Parkinson's disease dementia: Analysis of baseline data.

Halbgebauer S, Nagl M, Klafki H, Haußmann U, Steinacker P, Oeckl P, Kassubek J, Pinkhardt E, Ludolph AC, Soininen H, Herukka SK, Wiltfang J, Otto M
Scientific reports 2016 May 17;6:26145

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Maggot chymotrypsin I from Lucilia sericata is resistant to endogenous wound protease inhibitors.

Telford G, Brown AP, Kind A, English JS, Pritchard DI
The British journal of dermatology 2011 Jan;164(1):192-6

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Serpin A1/alpha 1-Antitrypsin by Western Blot. Western blot shows human plasma and lysates of human lung tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A1/alpha 1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Serpin A1/alpha 1-Antitrypsin at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Serpin A1/alpha 1-Antitrypsin by Simple Western^TM. Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1/alpha 1-Antitrypsin at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serpin A1/alpha 1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Serpin A1/alpha 1-Antitrypsin in HepG2 Human Cell Line. Serpin A1/alpha 1-Antitrypsin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Serpin A1/alpha 1-Antitrypsin Monoclonal Antibody (Catalog # MAB1268) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.