TA500068

antibody from OriGene

Targeting: SERPINA1 A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500068

Provider

OriGene

Proper citation

OriGene Cat#TA500068, RRID:AB_1527741

Product name

SERPINA1 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2)

Antibody type

Monoclonal

Description

SERPINA1 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2)

Host

Mouse

Conjugate

Unconjugated

Epitope

SERPINA1

Isotype

IgG

Antibody clone number

OTI1C2

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (RC202082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500068)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC202082 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SERPINA1 antibody(TA500068), and then analyzed by flow cytometry.

Validation comment

FC