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LearnTA500376
antibody from OriGene
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- TA500376 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500376, RRID:AB_2185291
- Product name
- SERPINA1 mouse monoclonal antibody, clone OTI8E3 (formerly 8E3)
- Antibody type
- Monoclonal
- Description
- SERPINA1 mouse monoclonal antibody, clone OTI8E3 (formerly 8E3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SERPINA1
- Isotype
- IgG
- Antibody clone number
- OTI8E3
- Vial size
- 100 µl
- Concentration
- 0.71 mg/ml
Submitted references Identification of proteomic and metabolic signatures associated with chemoresistance of human epithelial ovarian cancer.
Wu W, Wang Q, Yin F, Yang Z, Zhang W, Gabra H, Li L
International journal of oncology 2016 Oct;49(4):1651-65
International journal of oncology 2016 Oct;49(4):1651-65
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINA1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (RC202082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation(IP) of SERPINA1 by using TrueMab monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SERPINA1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500376)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500376)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500376)
- Validation comment
- IHC
