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LearnV8013
antibody from NSJ Bioreagents
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- V8013 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant Alpha 1 Antitrypsin Antibody / AAT / SERPINA1
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant Alpha 1 Antitrypsin antibody is suitable for use in Immunohistochemistry/Western blot applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- AAT/3167R
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Store the recombinant Alpha 1 Antitrypsin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples with recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples with recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R). Expected molecular weight: ~47 kDa (unmodified), 52 kDa (glycosylated).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human lung tissue lysate with recombinant Alpha 1 Antitrypsin antibody. Expected molecular weight: 47-52 kDa depending on the level of glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast with recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R) as confirmation of integrity and purity.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free recombinant Alpha 1 Antitrypsin antibody (clone AAT/3167R) as confirmation of integrity and purity.
