PA5-26439
antibody from Invitrogen Antibodies
Targeting: SERPINA1
A1A, A1AT, AAT, alpha-1-antitrypsin, alpha1AT, PI, PI1
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [3]
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Validation data
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- Product number
- PA5-26439 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha-1 Antitrypsin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references SERPINA1 Peptides in Urine as A Potential Marker of Preeclampsia Severity.
Starodubtseva N, Nizyaeva N, Baev O, Bugrova A, Gapaeva M, Muminova K, Kononikhin A, Frankevich V, Nikolaev E, Sukhikh G
International journal of molecular sciences 2020 Jan 30;21(3)
International journal of molecular sciences 2020 Jan 30;21(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a SERPINA1 polyclonal antibody (Product # PA5-26439) in A2058 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 SERPINA1 expression in placenta samples in cases of preeclampsia. ( A )-( D) Placenta villi in PE in the group with no SERPINA1 peptides in urine, stained granules, and membrane expression were determined. Fibrinoid deposits were stained, as well. ( A , B ) x400; ( C , D ) x200. ( E )-( H ) Placenta villi in PE in the group with SERPINA1 peptides in urine; membrane expression was lost. Fibrinoid deposits were stained also. ( E ) x400; ( F ) x200; ( G ) x100; ( H ) x200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 SERPINA1 expression in placenta samples in cases of chronic arterial hypertension and uncomplicated pregnancy. ( A , B ) Placenta villi in CAH group; membrane staining predominated. Fibrinoid deposits were stained. ( A , B ) x400. ( C )-( H ) Placenta villi in uncomplicated full-term pregnancy. Weak staining of syncytiotrophoblast, predominantly membrane staining; fibrinoid deposits were not stained or weakly stained; ( C )-( F ) x400; ( G ) x200; ( H ) x100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Comparison of SERPINA1 expression in syncytiotrophoblast according to immunohistochemistry: ( A ) SERPINA1 granule sizes (mum); ( B ) the relative level of expression of SERPINA1 in the membrane and cytoplasm of syncytiotrophoblast. The results are given for the four study groups. *-- p < 0.5; **-- p < 0.01; ***-- p < 0.001.