Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-17273 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CBS Monoclonal Antibody (GT519)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, 293T, Raji, K562, mouse liver, rat liver.
- Antibody clone number
- GT519
- Concentration
- 0.97 mg/mL
Submitted references Diabetic nephropathy associates with deregulation of enzymes involved in kidney sulphur metabolism.
Uyy E, Suica VI, Boteanu RM, Safciuc F, Cerveanu-Hogas A, Ivan L, Stavaru C, Simionescu M, Antohe F
Journal of cellular and molecular medicine 2020 Oct;24(20):12131-12140
Journal of cellular and molecular medicine 2020 Oct;24(20):12131-12140
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CBS was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CBS Monoclonal Antibody (GT519) (Product # MA5-17273) at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CBS Monoclonal Antibody (GT519) detects CBS protein by Western blot analysis. A. 30 µg Raji whole cell lysate/extract. B. 30 µg K562 whole cell lysate/extract.7.5 % SDS-PAGE. CBS Monoclonal Antibody (GT519) (Product # MA5-17273) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CBS Monoclonal Antibody (GT519) detects CBS protein by Western blot analysis. A. 50 µg mouse liver lysate/extract.7.5 % SDS-PAGE. CBS Monoclonal Antibody (GT519) (Product # MA5-17273) dilution: 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CBS Monoclonal Antibody (GT519) detects CBS protein by Western blot analysis. A. 50 µg rat liver lysate/extract.7.5 % SDS-PAGE. CBS Monoclonal Antibody (GT519) (Product # MA5-17273) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-CBS Monoclonal Antibody (GT519) (Product # MA5-17273) and a band at 61 kDa corresponding to CBS was observed across the cell lines and tissues tested. OVCAR-3 was observed to be high expressing cell line when compared to SK-OV-3; Mouse, Rat Liver and Mouse Pancreas were observed to be high expressing tissues when compared to Mouse and Rat Heart as reported in the literature. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), HeLa (Lane 3), OVCAR-3 (Lane 4), SK-OV-3 (Lane 5), DU 145 (Lane 6), tissue extracts of Mouse Liver (Lane 7), Rat Liver (Lane 8), Mouse Pancreas (Lane 9), Mouse Heart (Lane 10) and Rat Heart (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CBS was performed in paraffin-embedded mouse kidney tissue using CBS Monoclonal Antibody (GT519) (Product # MA5-17273) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CBS was performed in paraffin-embedded rat liver tissue using CBS Monoclonal Antibody (GT519) (Product # MA5-17273) at a dilution of 1:250.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 Changes in protein abundance induced by diabetes implicated in KEGG sulphur metabolism pathway and H 2 S production. (A) The proteins involved in sulphate activation and degradation, in clearance and H 2 S endogenous production are separately represented. Note that the following enzymes are up-regulated in diabetic kidney of double transgenic ( dTg) samples vs wild-type (WT) renal homogenates: Papss2 (by ~1.7-fold, P
