Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-72505 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CBS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CBS in mouse kidney tissue and Ramos cell lysate using 35 µg of protein per lane. Samples were incubated with CBS polyclonal antibody (Product # PA5-72505).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CBS in 293 cells using confocal microscopy. Samples were treated with CBS H1 polyclonal antibody (Product # PA5-72505). Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) was used as a secondary antibody. Actin filaments were labeled with Alexa Fluor 555 Phalloidin (red) and DAPI was used to stain the cell nuclei (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CBS in paraffin-embedded human hepatocarcinoma tissue. Samples were incubated with CBS polyclonal antibody (Product # PA5-72505) followed by a peroxidase-conjugate secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CBS in Ramos cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with CBS polyclonal antibody (Product # PA5-72505) followed by a FITC conjugated goat anti-rabbit secondary antibody.