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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NBP1-42768 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-42768, RRID:AB_10005917
- Product name
- Rabbit Polyclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography. This antibody is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Submitted references Adipose tissue hypoxia induces inflammatory M1 polarity of macrophages in an HIF-1α-dependent and HIF-1α-independent manner in obese mice.
Fujisaka S, Usui I, Ikutani M, Aminuddin A, Takikawa A, Tsuneyama K, Mahmood A, Goda N, Nagai Y, Takatsu K, Tobe K
Diabetologia 2013 Jun;56(6):1403-12
Diabetologia 2013 Jun;56(6):1403-12
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 beta/IL-1F2 Antibody [Biotin] [NBP1-42768] - Lane 1: Mouse IL-1ß Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.
