Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-42767 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-42767, RRID:AB_10005916
- Product name
- Rabbit Polyclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- Ion exchange chromatography. This antibody is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Submitted references Propranolol diminished severity of rat EAE by enhancing immunoregulatory/protective properties of spinal cord microglia.
Rat hippocampal responses up to 90 days after a single nanoceria dose extends a hierarchical oxidative stress model for nanoparticle toxicity.
Adipose tissue hypoxia induces inflammatory M1 polarity of macrophages in an HIF-1α-dependent and HIF-1α-independent manner in obese mice.
Human respiratory syncytial virus viroporin SH: a viral recognition pathway used by the host to signal inflammasome activation.
Rhinovirus-induced calcium flux triggers NLRP3 and NLRC5 activation in bronchial cells.
Pilipović I, Stojić-Vukanić Z, Prijić I, Jasnić N, Leposavić G
Neurobiology of disease 2020 Feb;134:104665
Neurobiology of disease 2020 Feb;134:104665
Rat hippocampal responses up to 90 days after a single nanoceria dose extends a hierarchical oxidative stress model for nanoparticle toxicity.
Hardas SS, Sultana R, Warrier G, Dan M, Wu P, Grulke EA, Tseng MT, Unrine JM, Graham UM, Yokel RA, Butterfield DA
Nanotoxicology 2014 Aug;8 Suppl 1:155-66
Nanotoxicology 2014 Aug;8 Suppl 1:155-66
Adipose tissue hypoxia induces inflammatory M1 polarity of macrophages in an HIF-1α-dependent and HIF-1α-independent manner in obese mice.
Fujisaka S, Usui I, Ikutani M, Aminuddin A, Takikawa A, Tsuneyama K, Mahmood A, Goda N, Nagai Y, Takatsu K, Tobe K
Diabetologia 2013 Jun;56(6):1403-12
Diabetologia 2013 Jun;56(6):1403-12
Human respiratory syncytial virus viroporin SH: a viral recognition pathway used by the host to signal inflammasome activation.
Triantafilou K, Kar S, Vakakis E, Kotecha S, Triantafilou M
Thorax 2013 Jan;68(1):66-75
Thorax 2013 Jan;68(1):66-75
Rhinovirus-induced calcium flux triggers NLRP3 and NLRC5 activation in bronchial cells.
Triantafilou K, Kar S, van Kuppeveld FJ, Triantafilou M
American journal of respiratory cell and molecular biology 2013 Dec;49(6):923-34
American journal of respiratory cell and molecular biology 2013 Dec;49(6):923-34
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1Beta precursor molecule. Denatured IL-1Beta will have a 18 kDa band.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody Peroxidase conjugated at 1:1,000 for 30 min at RT Block: MB-070 for 30 min RT Predicted/Observed size: 18 kDa, 18 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the Biotin conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis of Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 2ug/ml 1.5 hour @ room T Secondary Ab: MACH 1 HRP POLYMER 1:50 45” RT