MAB6964

antibody from Novus Biologicals

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

MAB6964

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-1 beta/IL-1F2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-1 beta/IL-1F2 Propeptide in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1F7, rhIL-1F10, rhIL-18, rhIL-36 alpha, rhIL-36 beta, IL-36 gamma, rhIL-36Ra, recombinant mouse IL-1Ra, or the mature peptides of recombinant IL-1 beta from canine, cotton rat, equine, feline, guinea pig, human, mouse, porcine, rabbit, rat, or rhesus is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Levistilide A Ameliorates NLRP3 Expression Involving the Syk-p38/JNK Pathway and Peripheral Obliterans in Rats.

Guo H, Sun L, Ling S, Xu JW
Mediators of inflammation 2018;2018:7304096

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MicroRNA-9 Inhibits NLRP3 Inflammasome Activation in Human Atherosclerosis Inflammation Cell Models through the JAK1/STAT Signaling Pathway.

Wang Y, Han Z, Fan Y, Zhang J, Chen K, Gao L, Zeng H, Cao J, Wang C
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;41(4):1555-1571

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Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation.

Lendermon EA, Coon TA, Bednash JS, Weathington NM, McDyer JF, Mallampalli RK
Respiratory research 2017 Jun 28;18(1):131

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NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis.

Li WL, Hua LG, Qu P, Yan WH, Ming C, Jun YD, Yuan LD, Nan N
Archives of medical science : AMS 2016 Oct 1;12(5):950-958

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Propionibacterium acnes Induces IL-1β secretion via the NLRP3 inflammasome in human monocytes.

Qin M, Pirouz A, Kim MH, Krutzik SR, Garbán HJ, Kim J
The Journal of investigative dermatology 2014 Feb;134(2):381-388

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Unsaturated fatty acids prevent activation of NLRP3 inflammasome in human monocytes/macrophages.

L'homme L, Esser N, Riva L, Scheen A, Paquot N, Piette J, Legrand-Poels S
Journal of lipid research 2013 Nov;54(11):2998-3008

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human IL-1 beta/IL-1F2 Propeptide by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 80 nM PMA for 48 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL-1 beta/IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 beta/IL-1F2 Propeptide at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human IL-1 beta/IL-1F2 Propeptide by Simple Western^TM. Simple Western lane view shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS, loaded at 0.2 mg/mL. A specific band was detected for IL-1 beta/IL-1F2 Propeptide at approximately 40 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL-1 beta/IL-1F2 Propeptide Monoclonal Antibody (Catalog # MAB6964). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.