Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-401-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects mouse IL-1 beta/IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant cotton rat IL-1 beta and recombinant rhesus monkey IL-1 beta is observed, approximately 15% cross-reactivity with recombinant rat IL-1 beta and recombinant rabbit IL-1 beta is observed, and less than 10% cross-reactivity with recombinant canine IL-1 beta , recombinant equine IL-1 beta , recombinant guinea pig IL-1 beta , recombinant porcine IL-1 beta , recombinant feline IL-1 beta , and recombinant human IL-1 beta is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle.
PtdIns4P on dispersed trans-Golgi network mediates NLRP3 inflammasome activation.
A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-α, and IL-1β.
Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis.
Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
Nakazawa H, Ikeda K, Shinozaki S, Yasuhara S, Yu YM, Martyn JAJ, Tompkins RG, Yorozu T, Inoue S, Kaneki M
FEBS open bio 2019 Feb;9(2):348-363
FEBS open bio 2019 Feb;9(2):348-363
PtdIns4P on dispersed trans-Golgi network mediates NLRP3 inflammasome activation.
Chen J, Chen ZJ
Nature 2018 Dec;564(7734):71-76
Nature 2018 Dec;564(7734):71-76
A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
Coll RC, Robertson AA, Chae JJ, Higgins SC, Muñoz-Planillo R, Inserra MC, Vetter I, Dungan LS, Monks BG, Stutz A, Croker DE, Butler MS, Haneklaus M, Sutton CE, Núñez G, Latz E, Kastner DL, Mills KH, Masters SL, Schroder K, Cooper MA, O'Neill LA
Nature medicine 2015 Mar;21(3):248-55
Nature medicine 2015 Mar;21(3):248-55
P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.
Wang H, Hong LJ, Huang JY, Jiang Q, Tao RR, Tan C, Lu NN, Wang CK, Ahmed MM, Lu YM, Liu ZR, Shi WX, Lai EY, Wilcox CS, Han F
Cell research 2015 Jun;25(6):674-90
Cell research 2015 Jun;25(6):674-90
Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-α, and IL-1β.
Zhao Y, Xiao X, Frank SJ, Lin HY, Xia Y
American journal of physiology. Endocrinology and metabolism 2014 Jul 15;307(2):E186-98
American journal of physiology. Endocrinology and metabolism 2014 Jul 15;307(2):E186-98
Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis.
Reed JR, Leon RP, Hall MK, Schwertfeger KL
Breast cancer research : BCR 2009;11(2):R21
Breast cancer research : BCR 2009;11(2):R21
Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
Bone 2007 May;40(5):1276-83
Bone 2007 May;40(5):1276-83
The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
Genes & development 2006 Sep 15;20(18):2527-38
Genes & development 2006 Sep 15;20(18):2527-38
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse IL-1 beta/IL-1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 4 hours and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours. PVDF membrane was probed with µg/mL of Goat Anti-Mouse IL-1 beta/IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta/IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse IL-1 beta/IL-1F2 by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL-1 beta/IL-1F2 at approximately 40 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse IL-1 beta/IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Mouse IL-1 beta/IL-1F2 Antibody. Recombinant Mouse IL-1 beta/IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta/IL-1F2 (10 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta/IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA). The ND50 is typically 2-12 µg/mL in the presence of concanavalin A (1.25 µg/mL).