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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB6811-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects porcine IL-1 beta in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) IL-1 beta and recombinant mouse (rm) IL-1 beta is observed and no cross-reactivity with rhIL-1 alpha , rmIL-1 alpha , recombinant porcine IL-1 alpha or recombinant rat IL-1 alpha is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutralization by Porcine IL-1 beta/IL-1F2 Antibody. Recombinant Porcine IL-1 beta/IL-1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta/IL-1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB6811). The ND50 is typically 4-15 µg/mL in the presence of concanavalin A (1.25 µg/mL).
