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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
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- Product number
- MAB401-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-1 beta/IL-1F2 in ELISAs.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Mouse IL-1 beta/IL-1F2 Antibody. Recombinant Mouse IL-1 beta/IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta/IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB401). The ND50 is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).
