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Western blot
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- Antibody Data
- Antigen structure
- References [34]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-501-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Rat IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat IL-1 beta/IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL-1 beta is observed and less than 0.5% cross-reactivity with recombinant human IL-1 beta and recombinant porcine IL-1 beta is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q63264- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Anti-Inflammatory Action of Sitagliptin and Linagliptin in Doxorubicin Nephropathy.
Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection.
Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms.
Andrographolide relieved pathological pain generated by spared nerve injury model in mice.
Glial interleukin-1β upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats.
Anti-Inflammatory and Antinociceptive Effects of Ethyl Acetate Fraction of an Edible Red Macroalgae Sarcodia ceylanica.
Lymphatic Circulation Disseminates Bartonella Infection Into Bloodstream.
Microglia-derived IL-1β promotes chemokine expression by Müller cells and RPE in focal retinal degeneration.
In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues.
Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia.
Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration.
Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure.
Spinal cord injury induces a long-lasting upregulation of interleukin-1β in astrocytes around the central canal.
The spinal anti-inflammatory mechanism of motor cortex stimulation: cause of success and refractoriness in neuropathic pain?
Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.
Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
Inflammatory mediators induced by amyloid-beta in the retina and RPE in vivo: implications for inflammasome activation in age-related macular degeneration.
Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke.
Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1β-induced attenuation of Rho signaling pathways in rat astrocytes.
Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus.
Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway.
Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland.
Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
Single subcutaneous administration of chorionic gonadotropin to rats induces a rapid and transient increase in testicular expression of pro-inflammatory cytokines.
A dual role for apolipoprotein e in neuroinflammation: anti- and pro-inflammatory activity.
Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
Glucocorticoids play a fundamental role in protecting the brain during innate immune response.
Jo CH, Kim S, Park JS, Kim GH
Kidney & blood pressure research 2018;43(3):987-999
Kidney & blood pressure research 2018;43(3):987-999
Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection.
Lai HC, Lu CH, Wong CS, Lin BF, Chan SM, Kuo CY, Wu ZF
Journal of the Chinese Medical Association : JCMA 2018 Nov;81(11):955-963
Journal of the Chinese Medical Association : JCMA 2018 Nov;81(11):955-963
Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms.
Taoro-Gonzalez L, Arenas YM, Cabrera-Pastor A, Felipo V
Journal of neuroinflammation 2018 Feb 8;15(1):36
Journal of neuroinflammation 2018 Feb 8;15(1):36
Andrographolide relieved pathological pain generated by spared nerve injury model in mice.
Wang HC, Tsay HS, Shih HN, Chen YA, Chang KM, Agrawal DC, Huang S, Lin YL, Lee MJ
Pharmaceutical biology 2018 Dec;56(1):124-131
Pharmaceutical biology 2018 Dec;56(1):124-131
Glial interleukin-1β upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats.
Zhang P, Bi RY, Gan YH
Journal of neuroinflammation 2018 Apr 20;15(1):117
Journal of neuroinflammation 2018 Apr 20;15(1):117
Anti-Inflammatory and Antinociceptive Effects of Ethyl Acetate Fraction of an Edible Red Macroalgae Sarcodia ceylanica.
Shih CC, Hwang HR, Chang CI, Su HM, Chen PC, Kuo HM, Li PJ, Wang HD, Tsui KH, Lin YC, Huang SY, Wen ZH
International journal of molecular sciences 2017 Nov 17;18(11)
International journal of molecular sciences 2017 Nov 17;18(11)
Lymphatic Circulation Disseminates Bartonella Infection Into Bloodstream.
Hong J, Li Y, Hua X, Bai Y, Wang C, Zhu C, Du Y, Yang Z, Yuan C
The Journal of infectious diseases 2017 Jan 15;215(2):303-311
The Journal of infectious diseases 2017 Jan 15;215(2):303-311
Microglia-derived IL-1β promotes chemokine expression by Müller cells and RPE in focal retinal degeneration.
Natoli R, Fernando N, Madigan M, Chu-Tan JA, Valter K, Provis J, Rutar M
Molecular neurodegeneration 2017 Apr 24;12(1):31
Molecular neurodegeneration 2017 Apr 24;12(1):31
In Vitro Neuroprotective and Anti-Inflammatory Activities of Natural and Semi-Synthetic Spirosteroid Analogues.
García-Pupo L, Zaldo-Castro A, Exarchou V, Tacoronte-Morales JE, Pieters L, Vanden Berghe W, Nuñez-Figueredo Y, Delgado-Hernández R
Molecules (Basel, Switzerland) 2016 Jul 29;21(8)
Molecules (Basel, Switzerland) 2016 Jul 29;21(8)
Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia.
Hernandez-Rabaza V, Cabrera-Pastor A, Taoro-Gonzalez L, Gonzalez-Usano A, Agusti A, Balzano T, Llansola M, Felipo V
Journal of neuroinflammation 2016 Apr 18;13(1):83
Journal of neuroinflammation 2016 Apr 18;13(1):83
Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration.
Jiao H, Natoli R, Valter K, Provis JM, Rutar M
PloS one 2015;10(12):e0143952
PloS one 2015;10(12):e0143952
Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure.
Madeira MH, Elvas F, Boia R, Gonçalves FQ, Cunha RA, Ambrósio AF, Santiago AR
Journal of neuroinflammation 2015 Jun 10;12:115
Journal of neuroinflammation 2015 Jun 10;12:115
Spinal cord injury induces a long-lasting upregulation of interleukin-1β in astrocytes around the central canal.
Paniagua-Torija B, Arevalo-Martin A, Molina-Holgado E, Molina-Holgado F, Garcia-Ovejero D
Neuroscience 2015 Jan 22;284:283-9
Neuroscience 2015 Jan 22;284:283-9
The spinal anti-inflammatory mechanism of motor cortex stimulation: cause of success and refractoriness in neuropathic pain?
Silva GD, Lopes PS, Fonoff ET, Pagano RL
Journal of neuroinflammation 2015 Jan 20;12:10
Journal of neuroinflammation 2015 Jan 20;12:10
Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.
Fu HQ, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang TL
PloS one 2014;9(8):e106331
PloS one 2014;9(8):e106331
Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
Shigemoto-Mogami Y, Hoshikawa K, Goldman JE, Sekino Y, Sato K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 5;34(6):2231-43
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 5;34(6):2231-43
Inflammatory mediators induced by amyloid-beta in the retina and RPE in vivo: implications for inflammasome activation in age-related macular degeneration.
Liu RT, Gao J, Cao S, Sandhu N, Cui JZ, Chou CL, Fang E, Matsubara JA
Investigative ophthalmology & visual science 2013 Mar 1;54(3):2225-37
Investigative ophthalmology & visual science 2013 Mar 1;54(3):2225-37
Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
Wei T, Guo TZ, Li WW, Hou S, Kingery WS, Clark JD
Journal of neuroinflammation 2012 Jul 23;9:181
Journal of neuroinflammation 2012 Jul 23;9:181
Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke.
Faustino JV, Wang X, Johnson CE, Klibanov A, Derugin N, Wendland MF, Vexler ZS
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Sep 7;31(36):12992-3001
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Sep 7;31(36):12992-3001
Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
Journal of neurochemistry 2011 Jan;116(2):217-26
Journal of neurochemistry 2011 Jan;116(2):217-26
Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1β-induced attenuation of Rho signaling pathways in rat astrocytes.
Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F
Journal of neurochemistry 2011 Apr;117(1):164-74
Journal of neurochemistry 2011 Apr;117(1):164-74
Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus.
Summy-Long JY, Hu S
American journal of physiology. Regulatory, integrative and comparative physiology 2009 Nov;297(5):R1532-45
American journal of physiology. Regulatory, integrative and comparative physiology 2009 Nov;297(5):R1532-45
Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
Lim G, Wang S, Zhang Y, Tian Y, Mao J
The Journal of clinical investigation 2009 Feb;119(2):295-304
The Journal of clinical investigation 2009 Feb;119(2):295-304
IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway.
Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H
Biochemical and biophysical research communications 2008 Jan 11;365(2):375-80
Biochemical and biophysical research communications 2008 Jan 11;365(2):375-80
Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
Infection and immunity 2008 Aug;76(8):3577-86
Infection and immunity 2008 Aug;76(8):3577-86
Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland.
Engström L, Rosén K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A
Endocrinology 2008 Apr;149(4):1436-50
Endocrinology 2008 Apr;149(4):1436-50
Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B
American journal of physiology. Heart and circulatory physiology 2007 Sep;293(3):H1839-46
American journal of physiology. Heart and circulatory physiology 2007 Sep;293(3):H1839-46
Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
Barkho BZ, Song H, Aimone JB, Smrt RD, Kuwabara T, Nakashima K, Gage FH, Zhao X
Stem cells and development 2006 Jun;15(3):407-21
Stem cells and development 2006 Jun;15(3):407-21
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Roche M, Diamond M, Kelly JP, Finn DP
Journal of neuroimmunology 2006 Dec;181(1-2):57-67
Journal of neuroimmunology 2006 Dec;181(1-2):57-67
Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S
Neuroscience 2005;132(3):625-31
Neuroscience 2005;132(3):625-31
Single subcutaneous administration of chorionic gonadotropin to rats induces a rapid and transient increase in testicular expression of pro-inflammatory cytokines.
Assmus M, Svechnikov K, von Euler M, Setchell B, Sultana T, Zetterström C, Holst M, Kiess W, Söder O
Pediatric research 2005 Jun;57(6):896-901
Pediatric research 2005 Jun;57(6):896-901
A dual role for apolipoprotein e in neuroinflammation: anti- and pro-inflammatory activity.
Guo L, LaDu MJ, Van Eldik LJ
Journal of molecular neuroscience : MN 2004;23(3):205-12
Journal of molecular neuroscience : MN 2004;23(3):205-12
Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
Nedrebø T, Reed RK, Jonsson R, Berg A, Wiig H
The Journal of physiology 2004 Apr 1;556(Pt 1):193-202
The Journal of physiology 2004 Apr 1;556(Pt 1):193-202
Glucocorticoids play a fundamental role in protecting the brain during innate immune response.
Nadeau S, Rivest S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Jul 2;23(13):5536-44
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Jul 2;23(13):5536-44
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 beta/IL-1F2 in Rat Splenocytes. IL-1 beta/IL-1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL-1 beta/IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutralization by Rat IL-1 beta/IL-1F2 Antibody. Recombinant Rat IL-1 beta/IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta/IL-1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-1 beta/IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND50 is typically 2-10 µg/mL in the presence of concanavalin A (1.25 µg/mL).
