Explore
Validate
Learn
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB8049 - Provider product page
- Provider
- R&D Systems
- Product name
- Equine IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects equine IL-1 beta in ELISAs.
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q28386- Isotype
- IgG
- Antibody clone number
- 855403
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Equine IL-1 beta/IL-1F2 Antibody. Recombinant Equine IL-1 beta/IL-1F2 induces proliferation in the D10.G4.1 mouse helper T cell line in the presence of concanavalin A (1.25 μg/mL) in a dose-depend-ent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-1 beta/IL-1F2 is neutralized (green line) by increasing concen-trations of Mouse Anti-Equine IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB8049). The ND50 is typically 2-10 μg/mL.
