Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB681 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects porcine IL-1 beta/IL-1F2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 50-100% cross-reactivity with IL-1 beta from human, mouse, and rat is observed.
- Reactivity
- Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P26889.- Isotype
- IgG
- Antibody clone number
- 77720
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic Escherichia coli.
Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic Escherichia coli.
Systemic cytokine profile in feeder pigs suffering from natural postweaning multisystemic wasting syndrome (PMWS) as determined by semiquantitative RT-PCR and flow cytometric intracellular cytokine detection.
Hypoxia-induced changes in tight junction permeability of brain capillary endothelial cells are associated with IL-1beta and nitric oxide.
Splíchalová A, Splíchal I, Trebichavský I, Hojná H
Folia microbiologica 2004;49(6):751-6
Folia microbiologica 2004;49(6):751-6
Expression of inflammatory markers in pig amnion after intraamniotic infection with nonpathogenic or enteropathogenic Escherichia coli.
Splíchalová A, Splíchal I, Trebichavský I, Hojná H
Folia microbiologica 2004;49(6):751-6
Folia microbiologica 2004;49(6):751-6
Systemic cytokine profile in feeder pigs suffering from natural postweaning multisystemic wasting syndrome (PMWS) as determined by semiquantitative RT-PCR and flow cytometric intracellular cytokine detection.
Sipos W, Duvigneau JC, Willheim M, Schilcher F, Hartl RT, Hofbauer G, Exel B, Pietschmann P, Schmoll F
Veterinary immunology and immunopathology 2004 May;99(1-2):63-71
Veterinary immunology and immunopathology 2004 May;99(1-2):63-71
Hypoxia-induced changes in tight junction permeability of brain capillary endothelial cells are associated with IL-1beta and nitric oxide.
Yamagata K, Tagami M, Takenaga F, Yamori Y, Itoh S
Neurobiology of disease 2004 Dec;17(3):491-9
Neurobiology of disease 2004 Dec;17(3):491-9
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Porcine IL-1 beta/IL-1F2 Antibody. Recombinant Porcine IL-1 beta/IL-1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta/IL-1F2 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB681). The ND50 is typically 1-3 µg/mL in the presence of concanavalin A (1.25 µg/mL).
