Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB33401 - Provider product page
- Provider
- R&D Systems
- Product name
- Equine IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects equine IL-1 beta/IL-1F2 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant canine, guinea pig, mouse, rat, and porcine IL-1 beta is observed and 10-50% cross-reactivity with recombinant feline, human, rabbit, and rhesus IL-1 beta is observed. Approximately 20% cross-reactivity with recombinant mouse (rm) IL-36 alpha and no cross-reactivity with recombinant human (rh) IL-36 alpha, rhIL-36 gamma, rhIL-1F10, rhIL-36Ra, rmIL-1Ra, rmIL-18, or rmIL-36 beta is observed.
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q28286
- Isotype
- IgG
- Antibody clone number
- 608714
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 beta/IL-1F2 in Equine PBMCs. IL-1 beta/IL-1F2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Equine IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB33401) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.