AB-401-NA

antibody from R&D Systems

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-401-NA

Provider

R&D Systems

Product name

Mouse IL-1 beta/IL-1F2 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects mouse IL-1 beta/IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant cotton rat IL-1 beta and recombinant rhesus monkey IL-1 beta is observed, approximately 15% cross-reactivity with recombinant rat IL-1 beta and recombinant rabbit IL-1 beta is observed, and less than 10% cross-reactivity with recombinant canine IL-1 beta , recombinant equine IL-1 beta , recombinant guinea pig IL-1 beta , recombinant porcine IL-1 beta , recombinant feline IL-1 beta , and recombinant human IL-1 beta is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P10749


Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle.

Nakazawa H, Ikeda K, Shinozaki S, Yasuhara S, Yu YM, Martyn JAJ, Tompkins RG, Yorozu T, Inoue S, Kaneki M
FEBS open bio 2019 Feb;9(2):348-363

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PtdIns4P on dispersed trans-Golgi network mediates NLRP3 inflammasome activation.

Chen J, Chen ZJ
Nature 2018 Dec;564(7734):71-76

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A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.

Coll RC, Robertson AA, Chae JJ, Higgins SC, Muñoz-Planillo R, Inserra MC, Vetter I, Dungan LS, Monks BG, Stutz A, Croker DE, Butler MS, Haneklaus M, Sutton CE, Núñez G, Latz E, Kastner DL, Mills KH, Masters SL, Schroder K, Cooper MA, O'Neill LA
Nature medicine 2015 Mar;21(3):248-55

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P2RX7 sensitizes Mac-1/ICAM-1-dependent leukocyte-endothelial adhesion and promotes neurovascular injury during septic encephalopathy.

Wang H, Hong LJ, Huang JY, Jiang Q, Tao RR, Tan C, Lu NN, Wang CK, Ahmed MM, Lu YM, Liu ZR, Shi WX, Lai EY, Wilcox CS, Han F
Cell research 2015 Jun;25(6):674-90

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Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-α, and IL-1β.

Zhao Y, Xiao X, Frank SJ, Lin HY, Xia Y
American journal of physiology. Endocrinology and metabolism 2014 Jul 15;307(2):E186-98

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Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis.

Reed JR, Leon RP, Hall MK, Schwertfeger KL
Breast cancer research : BCR 2009;11(2):R21

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Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.

Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
Bone 2007 May;40(5):1276-83

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The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.

Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
Genes & development 2006 Sep 15;20(18):2527-38

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse IL-1 beta/IL-1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 4 hours and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours. PVDF membrane was probed with µg/mL of Goat Anti-Mouse IL-1 beta/IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1 beta/IL-1F2 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse IL-1 beta/IL-1F2 by Simple Western^TM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 24 hours, loaded at 0.5 mg/mL. A specific band was detected for IL-1 beta/IL-1F2 at approximately 40 kDa (as indicated) using 50 µg/mL of Goat Anti-Mouse IL-1 beta/IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Mouse IL-1 beta/IL-1F2 Antibody. Recombinant Mouse IL-1 beta/IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta/IL-1F2 (10 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-1 beta/IL-1F2 Polyclonal Antibody (Catalog # AB-401-NA). The ND₅₀ is typically 2-12 µg/mL in the presence of concanavalin A (1.25 µg/mL).