F51185

antibody from NSJ Bioreagents

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F51185

Provider

NSJ Bioreagents

Product name

IL-1B Antibody

Antibody type

Polyclonal

Description

This highly specific IL-1B antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/ELISA applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the IL-1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of IL-1B antibody and NCI-H460 lysate. Predicted molecular weight: 31 kDa (precursor) and 17 kDa (mature).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human lung carcinoma with IL-1B antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of NCI-H292 cells using IL-1B antibody (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.