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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA319453 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-IL1 beta antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-IL1 beta antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- Il1b
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1? containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1? precursor molecule. Denatured IL-1? will have a 18 kDa band.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF after staining of mouse carotid artery tissue with anti-Mouse IL-1? antiserum (less purified form of TA319453) diluted 1:50. Panel A) shows no antibody staining of WT uninjured mouse carotid tissue. Panel B) shows anti-IL-1? staining of cells after surgical injury of tissue. Panel C) shows no antibody staining of injured carotid tissue from an IL-1? KO mouse.
- Validation comment
- IF
