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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Product number
- AF37471-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine IL-1 beta/IL-1F2 beta in direct ELISAs.
- Reactivity
- Canine
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Canine IL-1 beta/IL-1F2 Antibody. Recombinant Canine IL-1 beta/IL-1F2 (Catalog # 3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Proliferation elicited by Recombinant Canine IL-1 beta/IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Canine IL-1 beta/IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF37471). The ND50 is typically 3-18 ng/mL.
