Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB8406-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human IL-1 beta/IL-1F2 in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P01584
- Isotype
- IgG
- Antibody clone number
- 1027B
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-1 beta/ IL-1F2 in Human blood monocytes by Flow Cytometry. Human peripheral blood monocytes, either (A) untreated, or (B) treated with 500 ng/mL LPS overnight, were stained with Rabbit anti-Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB8406) followed by APC-conjugated anti-Rabbit IgG secondary antibody (Catalog # F0111) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on isotype control antibody staining (Catalog # MAB1050, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.