A00101-2

antibody from Boster Biological Technology

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00101-2

Provider

Boster Biological Technology

Product name

Anti-IL-1 beta Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for IL1B detection. Tested positive for ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot, in human, primate, dog

Reactivity

Human

Host

Rabbit

Vial size

1mg

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of IL1B using anti-IL1B antibody (A00101-2).IL1B was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL1B Antibody (A00101-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image