A00101-2

antibody from Boster Biological Technology

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00101-2

Provider

Boster Biological Technology

Product name

Anti-IL-1 beta Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal antibody for IL1B detection. Tested positive for ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot, in human, primate, dog

Reactivity

Human

Host

Rabbit

Vial size

1mg

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

References

Submitted references

A Synbiotic Ameliorates Con A-Induced Autoimmune Hepatitis in Mice through Modulation of Gut Microbiota and Immune Imbalance.

Liu Q, Yang H, Kang X, Tian H, Kang Y, Li L, Yang X, Liu H, Ren P, Kuang X, Tong M, Fan W
Molecular nutrition & food research 2023 Apr;67(7):e2200428

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Immune imbalance is associated with the development of preeclampsia.

Ma Y, Ye Y, Zhang J, Ruan CC, Gao PJ
Medicine 2019 Apr;98(14):e15080

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Interleukin-1 beta of Red nucleus involved in the development of allodynia in spared nerve injury rats.

Wang Z, Wang J, Li X, Yuan Y, Fan G
Experimental brain research 2008 Jul;188(3):379-84

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of IL1B using anti-IL1B antibody (A00101-2).IL1B was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL1B Antibody (A00101-2) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image