ABIN116862

antibody from antibodies-online

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Functional assay (Recommended by provider)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

ABIN116862

Provider

antibodies-online

Product name

anti-Interleukin 1, beta (IL1B) antibody

Antibody type

Polyclonal

Antigen

The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant 153 aa human IL1 beta produced in E.coli. Remarks: The MW of the recombinant IL-1b was 17,000 with the N-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL1 beta converting enzyme (ICE, capase-1).

Description

DEAE fractionation.

Reactivity

Human, Simian

Host

Rabbit

Vial size

1 mg

Concentration

2.0 mg/mL (by UV absorbance at 280 nm)

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

References

Submitted references

Sandwich ELISA formats designed to detect 17 kDa IL-1 beta significantly underestimate 35 kDa IL-1 beta.

Herzyk DJ, Berger AE, Allen JN, Wewers MD
Journal of immunological methods 1992 Apr 8;148(1-2):243-54

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Homogeneous interferon-inducing 22K factor is related to endogenous pyrogen and interleukin-1.

Van Damme J, De Ley M, Opdenakker G, Billiau A, De Somer P, Van Beeumen J
Nature 1985 Mar 21-27;314(6008):266-8

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Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.

Auron PE, Webb AC, Rosenwasser LJ, Mucci SF, Rich A, Wolff SM, Dinarello CA
Proceedings of the National Academy of Sciences of the United States of America 1984 Dec;81(24):7907-11