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- Antibody Data
- Antigen structure
- References [10]
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- Product number
- M420BB - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-1 beta Monoclonal Antibody (ILB1-H6), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M420BB targets IL-1 beta in ELISA applications and shows reactivity with Human samples. The M420BB immunogen is recombinant human IL-1 beta. M420BB detects IL-1 beta which has a predicted molecular weight of approximately 31 kDa. The M420BB IL1b antibody (biotinylated conjugate of clone ILB1-H6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M421B (clone ILB1-H67). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection. Biotinylated antibody M420BB (clone ILB1-H6) and antibody M421B (clone ILB1-H67) have successfully been used in combination with recombinant IL1 beta protein SIL1B in ELISA applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- ILB1-H6
- Vial size
- 250 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C
Submitted references Human eosinophils release IL-1ß and increase expression of IL-17A in activated CD4+ T lymphocytes.
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.
Protein microarray platform for the multiplex analysis of biomarkers in human sera.
Determination of the inflammatory potential of bioaerosols from a duck-fattening unit by using a limulus amebocyte lysate assay and human whole blood cytokine response.
Relationship between antipyretic effects and cytokine levels in uncomplicated falciparum malaria during different treatment regimes.
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
Essential role for Ca2+ in regulation of IL-1beta secretion by P2X7 nucleotide receptor in monocytes, macrophages, and HEK-293 cells.
HIV-1 coat protein gp120 stimulates interleukin-1beta secretion from human neuroblastoma cells: evidence for a role in the mechanism of cell death.
Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
Esnault S, Kelly EA, Nettenstrom LM, Cook EB, Seroogy CM, Jarjour NN
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2012 Dec;42(12):1756-64
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2012 Dec;42(12):1756-64
Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer's disease.
Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M
Neurobiology of aging 2010 Oct;31(10):1732-42
Neurobiology of aging 2010 Oct;31(10):1732-42
Protein microarray platform for the multiplex analysis of biomarkers in human sera.
Urbanowska T, Mangialaio S, Zickler C, Cheevapruk S, Hasler P, Regenass S, Legay F
Journal of immunological methods 2006 Oct 20;316(1-2):1-7
Journal of immunological methods 2006 Oct 20;316(1-2):1-7
Determination of the inflammatory potential of bioaerosols from a duck-fattening unit by using a limulus amebocyte lysate assay and human whole blood cytokine response.
Zucker BA, Scharf P, Kersten C
Journal of veterinary medicine. B, Infectious diseases and veterinary public health 2006 May;53(4):176-80
Journal of veterinary medicine. B, Infectious diseases and veterinary public health 2006 May;53(4):176-80
Relationship between antipyretic effects and cytokine levels in uncomplicated falciparum malaria during different treatment regimes.
Hugosson E, Montgomery SM, Premji Z, Troye-Blomberg M, Björkman A
Acta tropica 2006 Aug;99(1):75-82
Acta tropica 2006 Aug;99(1):75-82
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M
Rheumatology (Oxford, England) 2004 Aug;43(8):960-4
Rheumatology (Oxford, England) 2004 Aug;43(8):960-4
Essential role for Ca2+ in regulation of IL-1beta secretion by P2X7 nucleotide receptor in monocytes, macrophages, and HEK-293 cells.
Gudipaty L, Munetz J, Verhoef PA, Dubyak GR
American journal of physiology. Cell physiology 2003 Aug;285(2):C286-99
American journal of physiology. Cell physiology 2003 Aug;285(2):C286-99
HIV-1 coat protein gp120 stimulates interleukin-1beta secretion from human neuroblastoma cells: evidence for a role in the mechanism of cell death.
Corasaniti MT, Bilotta A, Strongoli MC, Navarra M, Bagetta G, Di Renzo G
British journal of pharmacology 2001 Nov;134(6):1344-50
British journal of pharmacology 2001 Nov;134(6):1344-50
Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
King CA, Marshall JS, Alshurafa H, Anderson R
Journal of virology 2000 Aug;74(15):7146-50
Journal of virology 2000 Aug;74(15):7146-50
Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.
King CA, Marshall JS, Alshurafa H, Anderson R
Journal of virology 2000 Aug;74(15):7146-50
Journal of virology 2000 Aug;74(15):7146-50
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA analysis of Mouse anti-Human IL-1beta biotinylated monoclonal antibody (Product # M420BB) was performed using the Thermo Scientific ELISA kit EH2IL1B by loading 50 µL per well of Human IL-1beta recombinant protein (Product # SIL1B) in quadruplicate at 400, 160, 64, 25.6, 10.24 and 0 pg/mL across a 5 µg/mL Mouse anti-Human IL-1beta (Product # M421B) pre-coated plate and incubated for 3 hours at room temperature. The plate was washed then incubated with 50 µL per well of Mouse anti-Human IL-1beta biotinylated antibody (Product # M420BB) in quadruplicate at 0.07 µg/mL for 3 hours at room temperature. The plate was washed and incubated with 100 µL per well of Ultra Streptavidin-HRP (Product # N504) in all test wells at 1:3000 for 30 minutes at room temperature. The plate was washed then incubated with 100 µL per well of Ultra TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin
