R1191P

antibody from Acris Antibodies GmbH

Targeting: IL1B IL-1B, IL1-BETA, IL1F2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Blocking/Neutralizing (Recommended by provider)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

R1191P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1191P, RRID:AB_10845328

Product name

anti Interleukin-1 beta / IL-1B

Antibody type

Polyclonal

Antigen

Recombinant Mouse IL-1 beta produced in E. coli

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

0.1 mg

Concentration

1.0 mg/ml (UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW Mouse IL-1beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL-1beta precursor molecule. Denatured IL-1beta will have a 18 kDa band.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Immunofluorescence Microscopy after staining of Mouse carotid artery tissue with anti-Mouse IL1-beta antiserum (Less purified form of R1191P) diluted 1/50. Tissue sections were prepared after cyrofixation. Reaction occurred at RT for 60' followed by washes and reaction with Rhodamine conjugated Goat anti Rabbit IgG (R1454T). Tissue was counterstained with bis-benzimide solution at 0.5 mg/ml in PBS for 15 min at RT. Panel A) shows no antibody staining of WT uninjured mouse carotid tissue. Panel B) shows anti-IL-1beta staining of cells after surgical injury of tissue. Panel C) shows no antibody staining of injured carotid tissue from an IL-1beta KO mouse.