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- Antibody Data
- Antigen structure
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- Product number
- MA5-17122 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP2 Monoclonal Antibody (4D1E2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17122 targets MMP2 in indirect ELISA and WB applications and shows reactivity with Human samples. The MA5-17122 immunogen is purified recombinant fragment of human MMP2 (amino acids: 242-396) expressed in E. Coli. MA5-17122 detects MMP2 which has a predicted molecular weight of approximately 73.8kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4D1E2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP2 using a MMP2 monoclonal antibody (Product # MA5-17122) against a human MMP2 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MMP2 Monoclonal Antibody (4D1E2) (Product # MA5-17122) and a 70kDa band corresponding to MMP2 was observed. Whole cell extracts (40 µg lysate) of U-87 MG (Lane 1), HT-1080 (Lane 2), LNCaP (Lane 3) and tissue extract (40 µg lysate) of Mouse Uterus (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Band of interest was not observed in LNCaP, a cell line that expresses very low levels of MMP2 (https://doi.org/10.1210/en.2002-0157).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP2 using a MMP2 monoclonal antibody (Product # MA5-17122) against a human MMP2 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP2 using MMP2 monoclonal antibody (Product # MA5-17122) in A431 (1) cell lysate.
