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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA1-16641 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP2 Monoclonal Antibody (MMP2/8B4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody is specific for both pro and active MMP-2.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MMP2/8B4
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Hypoxia Induces Mesenchymal Gene Expression in Renal Tubular Epithelial Cells: An in vitro Model of Kidney Transplant Fibrosis.
Zell S, Schmitt R, Witting S, Kreipe HH, Hussein K, Becker JU
Nephron extra 2013 Jan;3(1):50-8
Nephron extra 2013 Jan;3(1):50-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP2 in ProMMP2 (1), Active MMP2 (2) and human pressure ulcer biopsy (3). Samples were incubated in MMP2 monoclonal antibody (Product # MA1-16641).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MMP2 in formalin-fixed paraffin-embedded human ovary tissue. Samples were incubated in MMP2 monoclonal antibody (Product # MA1-16641) using a dilution of 2.5 µg/mL. Avidin-biotin-peroxidase detection method was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Representative Western blot of MMP2 in pre-miR-control (ctrl) and pre-miR-124-transfected 293 cells. The MMP2 protein level is reduced ~30% in cells overexpressing miR-124 compared to control cells. Cells were analyzed 4 days after transfection and three independent experiments were performed.
