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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [40]
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- Western blot [1]
- Other assay [4]
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- Product number
- MA5-14186 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP2 Monoclonal Antibody (VB3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-14186 targets MMP-2 (72kDa Collagenase IV) in IF, IP, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- VB3
- Concentration
- 0.2 mg/mL
Submitted references TAGLN and High-mobility Group AT-Hook 2 (HMGA2) Complex Regulates TGF-β-induced Colorectal Cancer Metastasis.
GnRH regulates trophoblast invasion via RUNX2-mediated MMP2/9 expression.
Intracranial extension of adenoid cystic carcinoma: potential involvement of EphA2 expression and epithelial-mesenchymal transition in tumor metastasis: a case report.
Expression of LGR8 and related biomarkers in hepatocellular carcinoma: correlation with clinicopathological parameters.
Expression of LGR8 and related biomarkers in hepatocellular carcinoma: correlation with clinicopathological parameters.
Expression of metalloproteases and their inhibitors by tumor and stromal cells in ductal carcinoma in situ of the breast and their relationship with microinvasive events.
Expression of matrix metalloproteinase (MMP)-2, MMP-9, and tissue inhibitor of MMP (TIMP)-1 in conjunctival melanomas and clinical implications.
Study of matrix metalloproteinases and their inhibitors in prostate cancer.
Expression of metalloproteases and their inhibitors in different histological types of breast cancer.
Immunohistochemical study of matrix metalloproteinases and their inhibitors in pure and mixed invasive and in situ ductal carcinomas of the breast.
Expression of metalloproteases and their inhibitors in primary tumors and in local recurrences after mastectomy for breast cancer.
Human mammary fibroblasts stimulate invasion of breast cancer cells in a three-dimensional culture and increase stroma development in mouse xenografts.
The expression of six biomarkers in the four most common ovarian cancers: correlation with clinicopathological parameters.
Protective effect of pomegranate-derived products on UVB-mediated damage in human reconstituted skin.
Inhibitory effect of CD147/HAb18 monoclonal antibody on cartilage erosion and synovitis in the SCID mouse model for rheumatoid arthritis.
Comparative analysis and clinical value of the expression of metalloproteases and their inhibitors by intratumor stromal fibroblasts and those at the invasive front of breast carcinomas.
Expression of urokinase plasminogen activator and its receptor in advanced epithelial ovarian cancer patients.
Phosphorylated hepatocyte growth factor receptor/c-Met is associated with tumor growth and prognosis in patients with bladder cancer: correlation with matrix metalloproteinase-2 and -7 and E-cadherin.
MMP-2, TIMP-1, E-cadherin, and beta-catenin expression in endometrial serous carcinoma compared with low-grade endometrial endometrioid carcinoma and proliferative endometrium.
Dominant negative LRP5 decreases tumorigenicity and metastasis of osteosarcoma in an animal model.
Quercetin inhibition of tumor invasion via suppressing PKC delta/ERK/AP-1-dependent matrix metalloproteinase-9 activation in breast carcinoma cells.
Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings.
Heme oxygenase-1 inhibits breast cancer invasion via suppressing the expression of matrix metalloproteinase-9.
Overexpression of matrix metalloproteinases and their inhibitors in mononuclear inflammatory cells in breast cancer correlates with metastasis-relapse.
Type IV collagen degradation in the myocardial basement membrane after unloading of the failing heart by a left ventricular assist device.
Matrix metalloproteinase-9 activity is associated with poor prognosis in T3-T4 node-negative colorectal cancer.
Matrix metalloproteinases and their inhibitors in the chamber angle of normal eyes and patients with primary open-angle glaucoma and exfoliation glaucoma.
Study of matrix metalloproteinases and their inhibitors in breast cancer.
Nicotine increases the collagen-degrading ability of human gingival fibroblasts.
A prospective, randomized, placebo-controlled, double-blinded, and split-face clinical study on LED phototherapy for skin rejuvenation: clinical, profilometric, histologic, ultrastructural, and biochemical evaluations and comparison of three different treatment settings.
EphA2 overexpression is associated with angiogenesis in ovarian cancer.
Effects of compressive loading on human synovium-derived cells.
Effects of compressive loading on human synovium-derived cells.
EMMPRIN-induced MMP-2 activation cascade in human cervical squamous cell carcinoma.
Nerve growth factor stimulates MMP-2 expression and activity and increases invasion by human pancreatic cancer cells.
Nerve growth factor stimulates MMP-2 expression and activity and increases invasion by human pancreatic cancer cells.
Overexpression of CEACAM6 promotes insulin-like growth factor I-induced pancreatic adenocarcinoma cellular invasiveness.
Ligation of EphA2 by Ephrin A1-Fc inhibits pancreatic adenocarcinoma cellular invasiveness.
Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations.
Mononuclear phagocyte differentiation, activation, and viral infection regulate matrix metalloproteinase expression: implications for human immunodeficiency virus type 1-associated dementia.
Zhou H, Li L, Xie W, Wu L, Lin Y, He X
OncoTargets and therapy 2020;13:10489-10498
OncoTargets and therapy 2020;13:10489-10498
GnRH regulates trophoblast invasion via RUNX2-mediated MMP2/9 expression.
Peng B, Zhu H, Klausen C, Ma L, Wang YL, Leung PC
Molecular human reproduction 2016 Feb;22(2):119-29
Molecular human reproduction 2016 Feb;22(2):119-29
Intracranial extension of adenoid cystic carcinoma: potential involvement of EphA2 expression and epithelial-mesenchymal transition in tumor metastasis: a case report.
Fukai J, Fujita K, Yamoto T, Sasaki T, Uematsu Y, Nakao N
BMC research notes 2014 Mar 7;7:131
BMC research notes 2014 Mar 7;7:131
Expression of LGR8 and related biomarkers in hepatocellular carcinoma: correlation with clinicopathological parameters.
Lin CK, Jin JS, Yu CP, Tsai WC
The Chinese journal of physiology 2011 Jun 30;54(3):161-8
The Chinese journal of physiology 2011 Jun 30;54(3):161-8
Expression of LGR8 and related biomarkers in hepatocellular carcinoma: correlation with clinicopathological parameters.
Lin CK, Jin JS, Yu CP, Tsai WC
The Chinese journal of physiology 2011 Jun 30;54(3):161-8
The Chinese journal of physiology 2011 Jun 30;54(3):161-8
Expression of metalloproteases and their inhibitors by tumor and stromal cells in ductal carcinoma in situ of the breast and their relationship with microinvasive events.
González LO, González-Reyes S, Junquera S, Marín L, González L, Del Casar JM, González JM, Vizoso F
Journal of cancer research and clinical oncology 2010 Sep;136(9):1313-21
Journal of cancer research and clinical oncology 2010 Sep;136(9):1313-21
Expression of matrix metalloproteinase (MMP)-2, MMP-9, and tissue inhibitor of MMP (TIMP)-1 in conjunctival melanomas and clinical implications.
Kim HK, Chae SW, Woo KI, Kim YD
Japanese journal of ophthalmology 2010 May;54(3):221-6
Japanese journal of ophthalmology 2010 May;54(3):221-6
Study of matrix metalloproteinases and their inhibitors in prostate cancer.
Escaff S, Fernández JM, González LO, Suárez A, González-Reyes S, González JM, Vizoso FJ
British journal of cancer 2010 Mar 2;102(5):922-9
British journal of cancer 2010 Mar 2;102(5):922-9
Expression of metalloproteases and their inhibitors in different histological types of breast cancer.
Del Casar JM, González-Reyes S, González LO, González JM, Junquera S, Bongera M, García MF, Andicoechea A, Serra C, Vizoso FJ
Journal of cancer research and clinical oncology 2010 Jun;136(6):811-9
Journal of cancer research and clinical oncology 2010 Jun;136(6):811-9
Immunohistochemical study of matrix metalloproteinases and their inhibitors in pure and mixed invasive and in situ ductal carcinomas of the breast.
Gonzalez LO, Junquera S, del Casar JM, González L, Marín L, González-Reyes S, Andicoechea A, González-Fernández R, González JM, Pérez-Fernández R, Vizoso FJ
Human pathology 2010 Jul;41(7):980-9
Human pathology 2010 Jul;41(7):980-9
Expression of metalloproteases and their inhibitors in primary tumors and in local recurrences after mastectomy for breast cancer.
del Casar JM, Carreño G, González LO, Junquera S, González-Reyes S, González JM, Bongera M, Merino AM, Vizoso FJ
Journal of cancer research and clinical oncology 2010 Jul;136(7):1049-58
Journal of cancer research and clinical oncology 2010 Jul;136(7):1049-58
Human mammary fibroblasts stimulate invasion of breast cancer cells in a three-dimensional culture and increase stroma development in mouse xenografts.
Olsen CJ, Moreira J, Lukanidin EM, Ambartsumian NS
BMC cancer 2010 Aug 19;10:444
BMC cancer 2010 Aug 19;10:444
The expression of six biomarkers in the four most common ovarian cancers: correlation with clinicopathological parameters.
Lin CK, Chao TK, Yu CP, Yu MH, Jin JS
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2009 Mar;117(3):162-75
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2009 Mar;117(3):162-75
Protective effect of pomegranate-derived products on UVB-mediated damage in human reconstituted skin.
Afaq F, Zaid MA, Khan N, Dreher M, Mukhtar H
Experimental dermatology 2009 Jun;18(6):553-61
Experimental dermatology 2009 Jun;18(6):553-61
Inhibitory effect of CD147/HAb18 monoclonal antibody on cartilage erosion and synovitis in the SCID mouse model for rheumatoid arthritis.
Jia J, Wang C, Shi Z, Zhao J, Jia Y, Zhao-Hui Z, Li X, Chen Z, Zhu P
Rheumatology (Oxford, England) 2009 Jul;48(7):721-6
Rheumatology (Oxford, England) 2009 Jul;48(7):721-6
Comparative analysis and clinical value of the expression of metalloproteases and their inhibitors by intratumor stromal fibroblasts and those at the invasive front of breast carcinomas.
Del Casar JM, González LO, Alvarez E, Junquera S, Marín L, González L, Bongera M, Vázquez J, Vizoso FJ
Breast cancer research and treatment 2009 Jul;116(1):39-52
Breast cancer research and treatment 2009 Jul;116(1):39-52
Expression of urokinase plasminogen activator and its receptor in advanced epithelial ovarian cancer patients.
Wang L, Madigan MC, Chen H, Liu F, Patterson KI, Beretov J, O'Brien PM, Li Y
Gynecologic oncology 2009 Aug;114(2):265-72
Gynecologic oncology 2009 Aug;114(2):265-72
Phosphorylated hepatocyte growth factor receptor/c-Met is associated with tumor growth and prognosis in patients with bladder cancer: correlation with matrix metalloproteinase-2 and -7 and E-cadherin.
Miyata Y, Sagara Y, Kanda S, Hayashi T, Kanetake H
Human pathology 2009 Apr;40(4):496-504
Human pathology 2009 Apr;40(4):496-504
MMP-2, TIMP-1, E-cadherin, and beta-catenin expression in endometrial serous carcinoma compared with low-grade endometrial endometrioid carcinoma and proliferative endometrium.
Shaco-Levy R, Sharabi S, Piura B, Sion-Vardy N
Acta obstetricia et gynecologica Scandinavica 2008;87(8):868-74
Acta obstetricia et gynecologica Scandinavica 2008;87(8):868-74
Dominant negative LRP5 decreases tumorigenicity and metastasis of osteosarcoma in an animal model.
Guo Y, Rubin EM, Xie J, Zi X, Hoang BH
Clinical orthopaedics and related research 2008 Sep;466(9):2039-45
Clinical orthopaedics and related research 2008 Sep;466(9):2039-45
Quercetin inhibition of tumor invasion via suppressing PKC delta/ERK/AP-1-dependent matrix metalloproteinase-9 activation in breast carcinoma cells.
Lin CW, Hou WC, Shen SC, Juan SH, Ko CH, Wang LM, Chen YC
Carcinogenesis 2008 Sep;29(9):1807-15
Carcinogenesis 2008 Sep;29(9):1807-15
Detection of migration of locally implanted AC133+ stem cells by cellular magnetic resonance imaging with histological findings.
Arbab AS, Janic B, Knight RA, Anderson SA, Pawelczyk E, Rad AM, Read EJ, Pandit SD, Frank JA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Sep;22(9):3234-46
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Sep;22(9):3234-46
Heme oxygenase-1 inhibits breast cancer invasion via suppressing the expression of matrix metalloproteinase-9.
Lin CW, Shen SC, Hou WC, Yang LY, Chen YC
Molecular cancer therapeutics 2008 May;7(5):1195-206
Molecular cancer therapeutics 2008 May;7(5):1195-206
Overexpression of matrix metalloproteinases and their inhibitors in mononuclear inflammatory cells in breast cancer correlates with metastasis-relapse.
González LO, Pidal I, Junquera S, Corte MD, Vázquez J, Rodríguez JC, Lamelas ML, Merino AM, García-Muñiz JL, Vizoso FJ
British journal of cancer 2007 Oct 8;97(7):957-63
British journal of cancer 2007 Oct 8;97(7):957-63
Type IV collagen degradation in the myocardial basement membrane after unloading of the failing heart by a left ventricular assist device.
Bruggink AH, van Oosterhout MF, de Jonge N, Cleutjens JP, van Wichen DF, van Kuik J, Tilanus MG, Gmelig-Meyling FH, van den Tweel JG, de Weger RA
Laboratory investigation; a journal of technical methods and pathology 2007 Nov;87(11):1125-37
Laboratory investigation; a journal of technical methods and pathology 2007 Nov;87(11):1125-37
Matrix metalloproteinase-9 activity is associated with poor prognosis in T3-T4 node-negative colorectal cancer.
Cho YB, Lee WY, Song SY, Shin HJ, Yun SH, Chun HK
Human pathology 2007 Nov;38(11):1603-10
Human pathology 2007 Nov;38(11):1603-10
Matrix metalloproteinases and their inhibitors in the chamber angle of normal eyes and patients with primary open-angle glaucoma and exfoliation glaucoma.
Rönkkö S, Rekonen P, Kaarniranta K, Puustjärvi T, Teräsvirta M, Uusitalo H
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2007 May;245(5):697-704
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2007 May;245(5):697-704
Study of matrix metalloproteinases and their inhibitors in breast cancer.
Vizoso FJ, González LO, Corte MD, Rodríguez JC, Vázquez J, Lamelas ML, Junquera S, Merino AM, García-Muñiz JL
British journal of cancer 2007 Mar 26;96(6):903-11
British journal of cancer 2007 Mar 26;96(6):903-11
Nicotine increases the collagen-degrading ability of human gingival fibroblasts.
Zhou J, Olson BL, Windsor LJ
Journal of periodontal research 2007 Jun;42(3):228-35
Journal of periodontal research 2007 Jun;42(3):228-35
A prospective, randomized, placebo-controlled, double-blinded, and split-face clinical study on LED phototherapy for skin rejuvenation: clinical, profilometric, histologic, ultrastructural, and biochemical evaluations and comparison of three different treatment settings.
Lee SY, Park KH, Choi JW, Kwon JK, Lee DR, Shin MS, Lee JS, You CE, Park MY
Journal of photochemistry and photobiology. B, Biology 2007 Jul 27;88(1):51-67
Journal of photochemistry and photobiology. B, Biology 2007 Jul 27;88(1):51-67
EphA2 overexpression is associated with angiogenesis in ovarian cancer.
Lin YG, Han LY, Kamat AA, Merritt WM, Landen CN, Deavers MT, Fletcher MS, Urbauer DL, Kinch MS, Sood AK
Cancer 2007 Jan 15;109(2):332-40
Cancer 2007 Jan 15;109(2):332-40
Effects of compressive loading on human synovium-derived cells.
Muroi Y, Kakudo K, Nakata K
Journal of dental research 2007 Aug;86(8):786-91
Journal of dental research 2007 Aug;86(8):786-91
Effects of compressive loading on human synovium-derived cells.
Muroi Y, Kakudo K, Nakata K
Journal of dental research 2007 Aug;86(8):786-91
Journal of dental research 2007 Aug;86(8):786-91
EMMPRIN-induced MMP-2 activation cascade in human cervical squamous cell carcinoma.
Sier CF, Zuidwijk K, Zijlmans HJ, Hanemaaijer R, Mulder-Stapel AA, Prins FA, Dreef EJ, Kenter GG, Fleuren GJ, Gorter A
International journal of cancer 2006 Jun 15;118(12):2991-8
International journal of cancer 2006 Jun 15;118(12):2991-8
Nerve growth factor stimulates MMP-2 expression and activity and increases invasion by human pancreatic cancer cells.
Okada Y, Eibl G, Guha S, Duffy JP, Reber HA, Hines OJ
Clinical & experimental metastasis 2004;21(4):285-92
Clinical & experimental metastasis 2004;21(4):285-92
Nerve growth factor stimulates MMP-2 expression and activity and increases invasion by human pancreatic cancer cells.
Okada Y, Eibl G, Guha S, Duffy JP, Reber HA, Hines OJ
Clinical & experimental metastasis 2004;21(4):285-92
Clinical & experimental metastasis 2004;21(4):285-92
Overexpression of CEACAM6 promotes insulin-like growth factor I-induced pancreatic adenocarcinoma cellular invasiveness.
Duxbury MS, Ito H, Benoit E, Zinner MJ, Ashley SW, Whang EE
Oncogene 2004 Jul 29;23(34):5834-42
Oncogene 2004 Jul 29;23(34):5834-42
Ligation of EphA2 by Ephrin A1-Fc inhibits pancreatic adenocarcinoma cellular invasiveness.
Duxbury MS, Ito H, Zinner MJ, Ashley SW, Whang EE
Biochemical and biophysical research communications 2004 Aug 6;320(4):1096-102
Biochemical and biophysical research communications 2004 Aug 6;320(4):1096-102
Abnormal expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in brain arteriovenous malformations.
Hashimoto T, Wen G, Lawton MT, Boudreau NJ, Bollen AW, Yang GY, Barbaro NM, Higashida RT, Dowd CF, Halbach VV, Young WL, University of California, San Francisco BAVM Study Group
Stroke 2003 Apr;34(4):925-31
Stroke 2003 Apr;34(4):925-31
Mononuclear phagocyte differentiation, activation, and viral infection regulate matrix metalloproteinase expression: implications for human immunodeficiency virus type 1-associated dementia.
Ghorpade A, Persidskaia R, Suryadevara R, Che M, Liu XJ, Persidsky Y, Gendelman HE
Journal of virology 2001 Jul;75(14):6572-83
Journal of virology 2001 Jul;75(14):6572-83
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of MMP-2 (72kDa Collagenase IV) using MMP-2 (72kDa Collagenase IV) Monoclonal Antibody (Product # MA5-14186) on HFL-1+TPA med Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MMP-2 (72kDa Collagenase IV) using MMP-2 (72kDa Collagenase IV) Monoclonal Antibody (Product # MA5-14186) on Native Human HFL-1+TPA med Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of MMP-2 (72kDa Collagenase IV) using MMP-2 (72kDa Collagenase IV) Monoclonal Antibody (Product # MA5-14186) on Native Human HFL-1+TPA med Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 TGF-beta-induced cell migration and invasion was reversed by knockdown of TAGLN . ( A ) Cell migration and invasion in HCT116 cells (left panel) and representative images of crystal violet staining (right panel). ( B ) Cell migration and invasion in HT29 cells (left panel) and representative images of crystal violet staining (right panel). ( C ) Representative images of Western blotting assay of E-cadherin, vimentin, fibronectin, MMP2, MMP9, and GADPH . 200 x magnification. * P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Overexpression of HMGA2 restored altered CRC cell migration and invasion induced by inhibition of TAGLN . ( A ) Cell migration and invasion in HCT116 cells (left panel) and representative images of crystal violet staining (right panel). ( B ) Cell migration and invasion in HT29 cells (left panel) and representative images of crystal violet staining (right panel). ( C ) Representative images of Western blotting assay of nucleus and total TAGLN levels. ( D ) Representative images of Western blotting assay of E-cadherin, vimentin, fibronectin, MMP2, MMP9, and GADPH . 200 x magnification. * P
