NB200-113

antibody from Novus Biologicals

Targeting: MMP2 CLG4, CLG4A, TBE-1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-113

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-113, RRID:AB_10000526

Product name

Mouse Monoclonal MMP-2 Antibody

Antibody type

Monoclonal

Description

Protein G purified. This is specific for pro and active MMP-2.

Reactivity

Human, Mouse, Rat, Guinea Pig

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Adipose-derived stem cells delay muscle atrophy after peripheral nerve injury in the rodent model.

Schilling BK, Schusterman MA 2nd, Kim DY, Repko AJ, Klett KC, Christ GJ, Marra KG
Muscle & nerve 2019 May;59(5):603-610

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Effects of acupuncture at GV20 and ST36 on the expression of matrix metalloproteinase 2, aquaporin 4, and aquaporin 9 in rats subjected to cerebral ischemia/reperfusion injury.

Xu H, Zhang Y, Sun H, Chen S, Wang F
PloS one 2014;9(5):e97488

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Exercise-induced regulation of matrix metalloproteinases in the skeletal muscle of subjects with type 2 diabetes.

Scheede-Bergdahl C, Bergdahl A, Schjerling P, Qvortrup K, Koskinen SO, Dela F
Diabetes & vascular disease research 2014 Sep;11(5):324-34

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Acetaminophen attenuates peroxynitrite-activated matrix metalloproteinase-2-mediated troponin I cleavage in the isolated guinea pig myocardium.

Rork TH, Hadzimichalis NM, Kappil MA, Merrill GF
Journal of molecular and cellular cardiology 2006 Apr;40(4):553-61

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Recombinant human MMP-2 expressed from CHO cells was was separated on a 7.5 % gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-MMP-2 in blocking buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Samples: 1) Human Recombinant MMP-2, and 2)Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - NB200-113, secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). MMP2 immunoreactivity was observed in the cytoplasm of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted MMP2). The cancer cells present in the core of tumor areas were found to be negative for MMP2 staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Analysis of a FFPE human breast cancer section using 5 ug/ml conc. of MMP2 antibody (clone 2C1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This MMP2 antibody generated an expected immunoreactivity of MMP2 protein in the cytoplasm of most of the cancer cells and the stromal cells, and in the inter-cellular spaces (secreted pool of MMP2). Staining was performed by Histowiz.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Upper image: MMP-2 FITC (green), DAPI (blue) Lower image: phase contrast of human choroid plexus (Alzheimer's disease). Image from verified customer review.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: MMP-2 Antibody (2C1) - (Pro and Active) [NB200-113] - Flow Cytometry: MMP-2 Antibody (2C1) [Alexa Fluor® 405] [NB200-113AF405] - CD4+ T cells were activated for 24h before being fixed and permeabilized to detect MMP-2. Image using the Alexa Fluor 405 form of this antibody. Image from verified customer review.