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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- GTX108587 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108587, RRID:AB_1952254
- Product name
- ZO-1 antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [C3], C-term (GTX108587) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE GTX108587 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ZO-1 antibody detects ZO-1 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extractC. 30 £gg HeLa whole cell lysate/extract 5 % SDS-PAGEZO-1 antibody (GTX108587) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [C3], C-term (GTX108587) diluted at 1:500.
