GTX108627

antibody from GeneTex

Targeting: TJP1 DKFZp686M05161, MGC133289, ZO-1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX108627

Provider

GeneTex

Proper citation

GeneTex Cat#GTX108627, RRID:AB_10731582

Product name

ZO-1 antibody [N2C1], Internal

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no

Proprietary.

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

Antibody Lot Number

40464

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: HCT116 5% SDS PAGE GTX108627 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ZO-1 antibody [N2C1], Internal detects TJP1 protein at junction by confocal immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: TJP1 protein stained by ZO-1 antibody [N2C1], Internal (GTX108627) diluted at 1:500.Blue: Hoechst 33342 staining.[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]